| 利用水稻基因组序列数据开发SSR标记的方法 |
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| 引用本文： | 朱宏波,方宣钧,杨仁崔. 利用水稻基因组序列数据开发SSR标记的方法[J]. 分子植物育种, 2003, 1(2): 273-276 |
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| 作者姓名： | 朱宏波 方宣钧 杨仁崔 |
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| 作者单位： | 1. 福建农林大学作物遗传育种研究所,福州,350002
2. 海南省热带农业资源开发利用研究所,三亚,572025 |
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| 摘 要： | 水稻基因组序列中存在着丰富的简单重复序列(SSR)，而已经开发利用的仅仅是一小部分。本文提出了通过搜索水稻基因组序列中的简单重复序列开发SSR标记的思路和方法，并介绍了应用本方法成功地在500kb范围内，获得22个SSR标记，其中10个在定位群体中扩增出多态带的SSR标记并将其定位，本实例验证了利用水稻基因组序列数据开发SSR标记是十分有效的。
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| 关 键 词： | 水稻 基因组序列 SSR标记 简单重复序列 开发 |
Developing SSR Markers Using Public Rice Genome Sequence Data |
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| Zhu Hongbo Fang Xuanjun Yang Rencui Fujian Agricultural and Forestry University,Fuzhou, Hainan Provincial Institute of Tropical Agricultural Resources,Sanya, Corresponding author,rcyang@pub fz fj cn. Developing SSR Markers Using Public Rice Genome Sequence Data[J]. Molecular Plant Breeding, 2003, 1(2): 273-276 |
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| Authors: | Zhu Hongbo Fang Xuanjun Yang Rencui Fujian Agricultural Forestry University Fuzhou Hainan Provincial Institute of Tropical Agricultural Resources Sanya Corresponding author rcyang@pub fz fj cn |
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| Affiliation: | Zhu Hongbo 1 Fang Xuanjun 2 Yang Rencui 1* 1 Fujian Agricultural and Forestry University,Fuzhou,350002 2 Hainan Provincial Institute of Tropical Agricultural Resources,Sanya,572025 * Corresponding author,rcyang@pub5 fz fj cn |
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| Abstract: | There are abundant SSRs in rice genome, a little part of them has been developed and utilized This paper has introduced the idea and method of how to devolop SSR markers by searching simple sequence repeats in rice genomes sequence data And also the authors introduced the case which successfully developped 22 SSR markers by searching the sequence data from the range of 500kb rice genome, 10 SSRs of them showed polymorphic bands in the mapping population and mapped in the chromosome This case proved that it is effective to develop SSR maker by using rice public genome sequence data |
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| Keywords: | Rice genomic sequence data Simple sequence repaet (SSR) |
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