| 道地药材茅苍术的ISSR分析 |
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| 引用本文： | 许梦云,吴沿友,赵玉国,杨晓勇.道地药材茅苍术的ISSR分析[J].河南农业科学,2009(7). |
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| 作者姓名： | 许梦云 吴沿友 赵玉国 杨晓勇 |
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| 作者单位： | 1. 现代农业装备与技术省部共建教育部重点实验室/江苏省重点实验室,江苏大学,农业工程研究院,江苏,镇江,212013
2. 现代农业装备与技术省部共建教育部重点实验室/江苏省重点实验室,江苏大学,农业工程研究院,江苏,镇江,212013;中国科学院,,地球化学研究所,环境地球化学国家重点实验室,贵州,贵阳,550002 |
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| 基金项目： | 江苏省高技术研究项目，镇江市农业项目 |
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| 摘 要： | 道地中药材茅苍术具有广泛的形态多样性。为了识别其遗传多样性,应用ISSR分子标记技术对16个茅苍术基因组和4个北苍术基因组进行了多态性分析。结果发现,茅苍术ISSR多态位点百分率为87.67%,北苍术ISSR多态位点百分率为53.33%,证明茅苍术比北苍术有更高的遗传多样性。采用SPSS13.0软件的Within-group linkage法对20个样品进行聚类分析。结果表明,茅苍术具有明显的遗传分化,且与北苍术有很近的亲缘关系。
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| 关 键 词： | 茅苍术 ISSR 遗传多样性 |
An ISSR Analysis on Famous Region-drug Atractylodes lancea |
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| XU Meng-yun,WU Yan-you,ZHAO Yu-guo,YANG Xiao-yong.An ISSR Analysis on Famous Region-drug Atractylodes lancea[J].Journal of Henan Agricultural Sciences,2009(7). |
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| Authors: | XU Meng-yun WU Yan-you ZHAO Yu-guo YANG Xiao-yong |
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| Abstract: | Atractylodes lancea, now in Chinese,is a very important famous region-drug. As a wild species,Atractylodes lancea is endemic to Jiangsu province.ISSR analysis was conducted on 20 samples (16 samples of Atractylis lancea genome and 4 samples of Atractylis chinensis genome)to investigate the genetic diversity.For Atractylis lancea,the polymorphic locus percentage is 87.67%,while for Atractylis chinensis,53.33%; which indicated that the levels of genetic diversity existed in Atractylodes lancea was higher than that in Atractylodes chinensis.Otherwise, the 20 samples were performed cluster analysis using within-group linkage method in SPSS 13.0 software.The analysis indicated that for Atractylis lancea there is obvious genetic differentiation,and there is close affiliation between Atractylodes lancea and Atractylodes chinensis. |
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| Keywords: | ISSR Atractylodes lancea ISSR Genetic diversity |
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