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| 怀地黄脱毒试管苗培养条件的优化 | |
| 引用本文： | 陈明霞,周彩云,李翔,刘好书,丁红娜,唐果,尚潇歌,张晓丽,李明军. 怀地黄脱毒试管苗培养条件的优化[J]. 河南农业科学, 2011, 40(5): 128-132 |
| 作者姓名： | 陈明霞 周彩云 李翔 刘好书 丁红娜 唐果 尚潇歌 张晓丽 李明军 |
| 作者单位： | 1. 河南师范大学,生命科学学院,河南新乡453007;河南省高校道地中药材保育及利用工程技术研究中心,河南新乡453007 2. 河南师范大学,生命科学学院,河南新乡453007 3. 河南师范大学,体育学院,河南新乡453007 4. 河南师范大学,生命科学学院,河南新乡453007;浚县实验中学,河南浚县456250 |
| 基金项目： | 国家农业科技成果转化资金项目，河南省教育厅科技攻关项目，河南师范大学青年科学基金，河南师范大学大学生创新性实验项目 |
| 摘 要： | 研究了怀地黄脱毒试管苗在不同pH值、不同光照强度和不同温度下形态指标的差异,以寻求怀地黄脱毒试管苗的最适培养条件。结果表明:适合培养怀地黄脱毒试管苗的pH值在5.8~6.2,该pH值范围内,试管苗生长较健壮,株高和繁殖系数最大,分别为6.66 cm和4.8。最适合培养怀地黄脱毒试管苗的光照强度为2 500 lx,此光照强度下,试管苗生长较健壮,叶色浓绿,株高和繁殖系数分别为5.2 cm和4.6。最适合培养怀地黄脱毒试管苗的环境温度值为30℃,该温度下试管苗生长较健壮,株高和繁殖系数最高,分别为5.8 cm和4.8。因此,适合怀地黄脱毒试管苗生长的pH值为5.8~6.2、光照强度为2 500 lx、温度为30℃。 |
| 关 键 词： | 怀地黄 脱毒试管苗 pH 光照强度 温度 |
Optimization for Culture Condition of Virus-free Plantlets of Rehmannia glutinosa f.Hueichingensis(Chanet Sehih) Hsiao. |
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| CHEN Ming-xia,ZHOU Cai-yun,LI Xiang,LIU Hao-shu,DING Hong-na,TANG Guo,SHANG Xiao-ge,ZHANG Xiao-li,LI Ming-jun. Optimization for Culture Condition of Virus-free Plantlets of Rehmannia glutinosa f.Hueichingensis(Chanet Sehih) Hsiao.[J]. Journal of Henan Agricultural Sciences, 2011, 40(5): 128-132 | |
| Authors: | CHEN Ming-xia ZHOU Cai-yun LI Xiang LIU Hao-shu DING Hong-na TANG Guo SHANG Xiao-ge ZHANG Xiao-li LI Ming-jun |
| Affiliation: | 1,2(1.College of Life Science,Henan Normal University,Xinxiang 453007,China;2.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese CrudeDrugs,University of Henan Province,Xinxiang 453007,China;3.College of Physical Education,Henan Normal University,Xinxiang 453007,China;4.Xunxian Experimental Middle School,Xunxian 456250,China) |
| Abstract: | In this paper,the morphological parameters of virus-free plantlets of Rehmannia glutinosa f.Hueichingensis(Chanet Sehih) Hsiao.were studied under different growing environment(pH value,different illumination intensity and different temperature) to find the optimum culture condition.The results were as follows:The optimum pH value was between 5.8 and 6.2,and the plantlets were healthy under pH5.8-6.2,the highest height and propagation coefficient were 6.66 cm and 4.8,respectively.The optimum illumination intensity was about 2 500 lx and plantlets were healthy under this illumination condition,the colour of leaves was deep green,plant height and propagation coefficient was 5.2 cm and 4.6,respectively.The best proper temperature was 30℃,and the plant height and propagation coefficient were 5.8 cm and 4.8,respectively.In a conclusion,the optimum condition for growth of virus-free plantlets of Rehmannia glutinosa f.Hueichingensis(Chanet Sehih) Hsiao.was:pH5.8-6.2,illumination intensity2 500 lx,and the proper temperature 30℃. |
| Keywords: | Rehmannia glutinosa f.Hueichingensis(Chan et Sehih) Hsiao. Virus-free plantlet pH value Illumination intensity Temperature |
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