| 中国板栗种质资源的AFLP分析 |
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| 引用本文： | 兰彦平,周连第,姚研武,王尚德,刘国彬.中国板栗种质资源的AFLP分析[J].园艺学报,2010,37(9):1499-1506. |
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| 作者姓名： | 兰彦平 周连第 姚研武 王尚德 刘国彬 |
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| 作者单位： | （北京市农林科学院农业综合发展研究所，北京 100097） |
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| 基金项目： | 北京市农林科学院常规育种财政专项，北京市科技新星计划项目 |
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| 摘 要： | 采用荧光标记AFLP技术，利用筛选出的9对EcoRⅠ/MseⅠ引物对90份中国板栗种质进行遗传变异及遗传关系分析。90份种质共产生806条带，695条多态性条带，多态比率86.23%；基于种质间遗传相似性系数的UPGMA聚类图将90份种质聚为两大类14个小组；9对引物在90个板栗种质中检测到数量不等的品种特异带型，各引物对对供试材料具有较高的鉴别效率，最高93%，最低80%。引物对两两组合可完全区分所有供试材料。
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| 关 键 词： | 板栗 亲缘关系 AFLP 种质鉴定 |
| 收稿时间： | 2010-03-09 |
Analysis of Castanea mollissima Germplasm Resources by AFLP |
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| LAN Yan-ping,ZHOU Lian-di,YAO Yan-wu,WANG Shang-de,LIU Guo-bin.Analysis of Castanea mollissima Germplasm Resources by AFLP[J].Acta Horticulturae Sinica,2010,37(9):1499-1506. |
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| Authors: | LAN Yan-ping ZHOU Lian-di YAO Yan-wu WANG Shang-de LIU Guo-bin |
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| Institution: | （Institute of Agricultural Integrated Development，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China） |
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| Abstract: | The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations. Among the 806 bands detected，695 bands were polymorphic，and the high percentage of polymorphic loci（86.23%）revealed a high genetic diversity among the 90 C. mollissima cultivars. The UPGMA phenogram based on genetic identity clustered the 90 C. mollissima cultivars into 2 groups and 14 rows. The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars，the highest ratio of identification is 93% and the lowest is 80%. |
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| Keywords: | AFLP |
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