| PRRSV重组N蛋白的抗原性分析及其特异性抗体制备 |
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| 引用本文： | 夏向荣,李玉峰,姜平. PRRSV重组N蛋白的抗原性分析及其特异性抗体制备[J]. 畜牧与兽医, 2003, 35(9): 6-8 |
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| 作者姓名： | 夏向荣 李玉峰 姜平 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 2 70 990 )，江苏省高新技术BG 2 0 0 2 31 7，江苏省攻关项目BE2 0 0 1 36 5 |
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| 摘 要： | 利用大肠杆菌表达技术和亲和层析法纯化出重组PRRSV N蛋白 ,用间接ELISA法和Western blot分析 ,证明其具有较好的抗原性。将该纯化蛋白采用大剂量长程免疫法免疫家兔 ,制备了兔抗重组N蛋白抗体 ,用Western blot分析和竞争抑制试验证明其具有很高的特异性 ,ELISA效价达 1∶32 0 0以上。
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| 关 键 词： | PRRSV 核衣壳蛋白 抗体 间接ELISA Westernblot |
| 文章编号： | 0529-5130(2003)09-0006-03 |
| 修稿时间： | 2003-03-10 |
Analysis of antigenicity and preparation of high specific antibody to recombinant N protein of PRRSV |
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| XIA Xiang rong,LI Yu feng,JIANG Ping. Analysis of antigenicity and preparation of high specific antibody to recombinant N protein of PRRSV[J]. Animal Husbandry & Veterinary Medicine, 2003, 35(9): 6-8 |
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| Authors: | XIA Xiang rong LI Yu feng JIANG Ping |
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| Abstract: | Recombinant N protein of PRRSV was expressed in E. coli expression system, and purified with affinity chromatography. It could specifically react with pig serum antibody to PRRS in indirect ELISA test and Western blotting.Through long span immunity with two rabbits, the serum antibody against N protein was prepared. After the antibody was treated with BL21, Its high specifity to N protein was verified by Western blotting and competitive ELISA test. The antibody titer was 1∶3 200 with ELISA test. |
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| Keywords: | PRRSV Recombinant nucleocapsid protein Antibody Indirect ELISA Western blot |
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