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中国樱桃泰山干樱的组织培养及植株再生研究
引用本文:魏海蓉,刘庆忠,艾呈祥,张力思,李国田. 中国樱桃泰山干樱的组织培养及植株再生研究[J]. 中国农学通报, 2009, 25(1): 39-41
作者姓名:魏海蓉  刘庆忠  艾呈祥  张力思  李国田
作者单位:山东省果树研究所,泰安,271000
基金项目:山东省农业科学院青年基金,山东省农业良种产业化工程项目 
摘    要:以中国樱桃泰山干樱(Prunus pseudocerasus Lindl.)一年生枝的休眠芽为外植体,研究了不同种类和不同浓度生长调节物质对泰山干樱初代培养、增殖和生根的影响。结果表明,泰山干樱的最佳初代培养基为MS+6-BA1.0mg/L+IBA0.2 mg/L;MS培养基中分别加入0.5mg/L 6-BA 和0.1mg/L ZT,增殖效果最好,增殖系数达6.8,有效新梢数可达80.2%;组培苗在1/2 MS+IBA 0.5mg/L培养基上根诱导效果最好,生根率可达80%,平均根长4.54cm;移栽到草炭土和河沙1∶1的混合基质中,温室炼苗后,成活率可达70%以上。

关 键 词:日光温室  日光温室  制约因素  可持续发展  
收稿时间:2008-08-21
修稿时间:2008-11-09

Tissue Culture and Rapid Propagation of Chinese Cherry Taishanganying(Prunus pseudocerasus Lindl.)
Wei Hairong,Liu Qingzhong,Ai Chengxiang,Zhang Lisi,Li Guotian. Tissue Culture and Rapid Propagation of Chinese Cherry Taishanganying(Prunus pseudocerasus Lindl.)[J]. Chinese Agricultural Science Bulletin, 2009, 25(1): 39-41
Authors:Wei Hairong  Liu Qingzhong  Ai Chengxiang  Zhang Lisi  Li Guotian
Affiliation:(Shandong Institute of Pomology, Tai' an 271000)
Abstract:Experiment was conducted with the dormancy buds of Taishanganying, the effect of plant growth regulators on the initial culture、multiplication and root regeneration were analyzed and studied. The results showed that on the medium of MS+6-BA1.0mg/L+IBA0.1 mg/L the best of clump shoot was inducted; MS medium with 0.5mg/L 6-BA and 0.1mg/L ZT was the most suitable for the propagation of Taishanganying and the propagation rate reached 6.8times.The effective number of new shoot was up to 80.2%.On the other hand,the good medium to induce root was 1/2 MS+IBA 0.5mg/Lwith 80% of rooting rate and 4.54cm of average root length. test-tube saplings having taken root were ransplanted in soil texture hased on river sand and turf.Small arch plastic shed in conservatory kept moisture and temperature ,the survival rate of transplant came up to more than 70%.
Keywords:Chinese cherry   tissue culture   proliferation   rooting
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