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| 一个水稻类病变黄叶突变体的鉴定和精细定位 | |
| 引用本文： | 吴超,付亚萍,胡国成,斯华敏,刘旭日,孙宗修,程式华,刘文真.一个水稻类病变黄叶突变体的鉴定和精细定位[J].中国水稻科学,2011,25(3):256-260. |
| 作者姓名： | 吴超 付亚萍 胡国成 斯华敏 刘旭日 孙宗修 程式华 刘文真 |
| 作者单位： | 1. 沈阳农业大学,农学院,辽宁,沈阳,110161;中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州,310006;浙江省农业科学院,园艺研究所,浙江,杭州,310021 2. 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州,310006 3. 沈阳农业大学,农学院,辽宁,沈阳,110161;中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州,310006 |
| 基金项目： | 国家自然科学基金资助项目，转基因生物新品种培育科技重大专项资助项目 |
| 摘 要： | 从15000多个水稻转基因株系中发现了一个类病变黄叶突变体.该突变体最显著的特征为叶片由下而上依次黄化,同时出现类似病原体感染的病斑.根据突变体表型,将该突变体命名为syll(spotted and yellow leayes 1).遗传学分析表明,该突变性状受1对隐性核基因控制.PCR检测和潮霉索抗性分析显示.该突变... |
| 关 键 词： | 水稻 类病变突变体 黄叶 分子标记 精细定位 |
| 收稿时间： | 1900-01-01; |
Identification and Fine Mapping of a Spotted and Yellow Leaf Mutant in Rice |
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| WU Chao,FU Ya-ping,HU Guo-cheng,SI Hua-min,LIU Xu-ri,SUN Zong-xiu,CHENG Shi-hua,LIU Wen-zhen.Identification and Fine Mapping of a Spotted and Yellow Leaf Mutant in Rice[J].Chinese Journal of Rice Science,2011,25(3):256-260. | |
| Authors: | WU Chao FU Ya-ping HU Guo-cheng SI Hua-min LIU Xu-ri SUN Zong-xiu CHENG Shi-hua LIU Wen-zhen |
| Institution: | WU Chao1,2,3,FU Ya-ping2,HU Guo-cheng2,SI Hua-min2,LIU Xu-ri2,SUN Zong-xiu2,CHENG Shi-hua1,LIU Wen-zhen2,(1 College of Agronomy,Shenyang Agricultural University,Shenyang 110161,China,2State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,3Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
| Abstract: | A spotted and yellow leaf mutant was isolated from more than 15 000 transgenic rice lines.The mutant was featured by lesion-mimic spots during the whole growth stage as well as leaf etiolation one by one from lower part to upper part.Genetic analysis revealed that the mutation was controlled by a single recessive gene,which was designated as syl1(spotted and yellow leaves 1).PCR analysis and hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion.To isolate the syl1 gene,a pos... |
| Keywords: | rice lesion mimic mutant yellow leaf molecular marker fine mapping |
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