无外源氮素条件下接种促生菌对箭筈豌豆生长及根系特性影响

利用前期筛选的优良促生菌株研制微生物接种剂,在无外源氮素条件下测定其对箭筈豌豆(Vicia sativa)生长及根系特性的影响。结果表明:无外源氮素条件下,接种研制的优良促生菌接种剂对箭筈豌豆地上及根系生长有显著促进作用,箭筈豌豆株高、生物量、部分根系形态学指标(根长、根表面积、根体积及根平均直径)以及根系活力等均显著提高。不同处理中,复合接种剂效果优于单一接种剂。复合接种剂中,处理D(根瘤菌+联合固氮菌+溶磷菌:G3+Y16+J3-1)效果最佳,其对箭筈豌豆株高、地上生物量、地下生物量、根长、根表面积、根体积、根平均直径及根系活力分别较对照增加45.75%,177.16%,211.69%,73.7%,238.9%, 199.2%,12.4%和44.2%。虽然单一根瘤菌剂(G3)表现出与D处理相近的促生效果, 但D处理对根系活力提高显著,所以推荐根瘤菌+联合固氮菌+溶磷菌株的复合接种剂作为后期研制箭筈豌豆生物菌肥的最佳菌株组合。

Effects of PGPR Inoculum without Exogenous Nitrogen on the Growth and Root System Characteristics of Vicia sativa L.

Three PGPR(Strain No.: G3, Y16, J3-1) strains were isolated and screened from the rhizosphere of vetch(Vicia sativa). Both single inoculum and compound inoculum were designed with these strains. Effect of inoculum on the growth and root system characteristics of vetch was determined by an indoor experiment. The results indicated that the plant height, biomass, diameter, root length and root activity of vetch increased after using inoculants. The effectiveness of treatment D (phosphate dissolving + nitrogen-fixing+rhizobia inoculants :G3+Y16+J3-1) was better than single inoculant. The plant height, aboveground biomass, underground biomass, root length, surface area, root volume, root diameter and root activity increased by 45.75%, 177.16%, 211.69%, 73.7%, 238.9%, 199.2%, 12.4% and 44.2%, respectively. Meanwhile, A(G3) was the best single inoculum. The compound inoculum (G3+Y16+J3-1) was recommended as the best resources to substitute rhizosphere inoculum for Vicia sativa.