养蚕环境泥土中家蚕核型多角体病毒的PCR检测技术研究

家蚕核型多角体病（又称血液型脓病）是家蚕病毒病中危害最为严重的，为了更好的防控此病，以泥土作为环境因子代表，探讨了用PCR方法检测环境中病原BmNPV的可行性。研究结果表明，以BmNPV保守基因polyhedrin为靶标基因，设计特异性引物，所建立的PCR检测方法能够成功检测到泥土中BmNPV；对泥土中另外3种昆虫的NPV进行PCR检测，未发现特异性扩增，表明该方法具有良好的特异性；敏感性试验结果表明能检测环境泥土中BmNPV多角体的个数为8.3×10⁵个，灵敏度是镜检的100倍。

Detection of Bombyx mori nucleopolyhedrovirus from Rearing Soil Using PCR

Bombyx mori nucleopolyhedrovirus disease is the most serious disease among silkworm virus diseases. In order to prevent and control this disease, using soil representing as environment factor, feasibility of detecting pathogen BmNPV in the environment by PCR was discussed. High conserved gene of polyhedrin of BmNPV was used to design specific primers and BmNPV in soil could be detected successfully by PCR. No specific amplification was found from the other three lepidopteran-specific nucleopolyhedrovirus in soil by PCR, indicating that this method was highly specific. Sensitivity test showed that viral polyhedra number was 8.3×10⁵ in the soil detected by PCR, and was 100-fold of that detected by microscopy.