‘大白谷’CC-NBS-LRR蛋白编码基因抗干尖线虫侵染时空表达研究

为深入探究‘大白谷’(Oryza sativa L.ssp.Indica)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了‘大白谷’CC-NBS-LRR蛋白编码基因Os11g RGA8,对Os11g RGA8基因进行了生物信息学分析,并对其在水稻抗干尖线虫侵染过程中的功能进行了研究。Os11g RGA8基因位于11号染色体,其编码蛋白含有733个氨基酸,等电点为5.99,相对分子质量为84355.07。序列分析表明该氨基酸序列含有RX-CC、AAA_22、NBS和LRR_4这4个结构域,Os11g RGA8基因编码抗病蛋白属于CC-NBSLRR抗病蛋白家族。Q-PCR结果显示,接种水稻干尖线虫SAMN02420038的籼型常规稻‘大白谷’,与CK组‘大白谷’相比,Os11g RGA8基因表达量表现为上调,且24 h内上调增幅较缓,48 h时上调增幅突然上涨,72 h时上调增幅又出现下降,表明该基因参与‘大白谷’天然免疫反应过程,在水稻干尖线虫侵染过程中起到一定抗性作用。

Spatial-temporal Expression of CC-NBS-LRR Protein-coding Gene Resisting to Infection of Aphelenchoides besseyi in Oryza sativa L. ssp. Indica

Os11gRGA8 gene was cloned and its bioinformatics were analyzed in order to explore the molecular mechanism of the indica rice (Oryza sativa L. ssp. Indica) and the role of resistance genes in the course of illness. Bioinformatics of Os11gRGA8 gene was analyzed, and its function in the process of Aphelenchoides besseyi infection was studied. Os11gRGA8 genes were in chromosome 11, containing 733 amino acids, its coding protein isoelectric point was 5.99 and the relative molecular mass was 84355.07. The sequence analysis showed that four structure domains RX-CC, AAA_22, NBS and LRR_4 were contained in its amino acid sequence and the disease-resistant protein encoded by Os11gRGA8 gene belonged to CC-NBS-LRR disease-resistant protein family. Q-PCR results showed that compared with CK rice seedlings group, the Indica inoculated by A.besseyi SAMN02420038 got a raised quantity of Os11gRGA8 gene expression and within 24 h the increase was relatively slow. Then the growth raised suddenly in 48 h, but the increase declined in 72 h. Indicated that Os11gRGA8 gene was involved in Indica natural immune reaction process and played a role in the process of A.besseyi resistance infection.