Irreversible competitive inhibitory kinetics of cardol triene on mushroom tyrosinase |
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Authors: | Zhuang Jiang-Xing Hu Yong-Hua Yang Mei-Hua Liu Feng-Jiao Qiu Ling Zhou Xing-Wang Chen Qing-Xi |
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Affiliation: | Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences,Xiamen University, Xiamen 361005, China. |
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Abstract: | Cardol triene was first purified from cashew (Anacardium occidentale L.) nut shell liquid and identified by gas chromatography coupled to mass spectroscopy and nuclear magnetic resonance. The effects of this compound on the activity of mushroom tyrosinase were studied. The results of the kinetic study showed that cardol triene was a potent irreversible competitive inhibitor and the inactivation was of the complexing type. Two molecules of cardol triene could bind to one molecule of tyrosinase and lead to the complete loss of its catalytic activity. The microscopic rate constants were determined for the reaction of cardol triene with the enzyme. The anti-tyrosinase kinetic research of this study provides a comprehensive understanding of inhibitory mechanisms of resorcinolic lipids and is beneficial for the future design of novel tyrosinase inhibitors. |
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