60Coγ辐照对霍山石斛悬浮培养原球茎生长和生物碱积累的影响

以霍山石斛原球茎为材料，研究5、10、20和30Gy的60Co γ辐照处理对原球茎悬浮培养生物碱积累的影响。结果表明，60Co γ辐照处理能提高悬浮培养原球茎生物碱含量，且适宜辐照剂量为10Gy。 10Gy处理的原球茎培养36d时，原球茎鲜重为26.54g/瓶，生物碱含量为0.035%，此时培养液pH和原球茎电导率变化较小，培养液环境适合原球茎继续培养；同时辐照处理可促进POD、SOD、CAT和PAL酶活性，抑制PPO酶活性，从而促进石斛原球茎生长和生物碱合成。

EFFECTS OF 60Co γ-RAYS ON CELL GROWTH AND ALKALOID ACCUMULATION OF PROTOCORM-LIKE BODIES IN SUSPENSION CULTURES FROM D.HUOSHANENSE

Protocorm-like bodies(PLBs) in suspension cultures from Dendrobium huoshanense were irradiated by 60Co γ-rays with different doses,such as 5Gy, 10Gy, 20Gy and 30Gy, and alkaloid accumulation of PLBs was studies.The results showed that 60Co γ-rays irradiation could improve the alkaloid content of PLBs, and the suitable dose was 10Gy. The fresh weight of 10Gy irradiated PLBs was 26.54g/flask, and the alkaloid content was 0.035% on the 36th day .The medium pH and electric conductivity of 10Gy irradiated PLBs changed slightly during the suspension culture period. The results suggested such cultural environment was suitable for PLBs growth .Results also show that 60Co γ-rays irradiation could increase the activities of POD, SOD, CAT, PAL and decrease the activity of PPO, such were responsible for the improvement of cell growth and alkaloid accumulation in PLBs.