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| 常规PCR和巢式PCR法鉴定牛早期胚胎性别体系的建立和优化 | |
| 引用本文： | 王世银,张兆旺,赵兴绪,张伟,赵建军.常规PCR和巢式PCR法鉴定牛早期胚胎性别体系的建立和优化[J].现代畜牧兽医,2008(4):53-56. |
| 作者姓名： | 王世银 张兆旺 赵兴绪 张伟 赵建军 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070 2. 甘肃农业大学动物医学院,甘肃,兰州,730070 3. 新疆农业职业技术学院动物科技学院,新疆,昌吉,831100 4. 西南农业大学,重庆荣昌,402460 |
| 摘 要： | 试验利用牛Y染色体重复序列作为雄性特异性引物,_以肿蔼坏死因子(TNFα)为内标引物建立多重PCR和多重巢式PCR体系,进行牛早期胚胎性别鉴定.共设计4对引物-Y染色体重复序列外引物和内引物,其扩增片段大小分别为534 bp和480bp,肿瘤坏死因子外引物和内引物,扩增片段大小分别为357 bp和272 bp.结果表明,4对引物均有很高的特异性和稳定性;多重PCR体系灵敏度为50 pg(约8个细胞),多重巢式PCR体系灵敏度为10 pg(约2个细胞),故多重巢式PCR体系更适合于牛胚胎性别鉴定. |
| 关 键 词： | 胚胎性别鉴定 牛 Y染色体重重复序列 PCR法 |
Establishing and Optimizing Conventional PCR and Nested PCR System for Sex Determination in Early Bovine Embryo |
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| Wang Shiyin,Zhang Zhaowang,Zhao Xingxu,Zhang Wei,Zhao Jianjun.Establishing and Optimizing Conventional PCR and Nested PCR System for Sex Determination in Early Bovine Embryo[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2008(4):53-56. | |
| Authors: | Wang Shiyin Zhang Zhaowang Zhao Xingxu Zhang Wei Zhao Jianjun |
| Abstract: | In this study,we designed four pairs of primers which the amplifiment products were 534bp,480bp,357bp and 272bp respectively length according to Y chromosome repeated sequence and tumor necrosis factor alpha(TNFa)for Sex Determination of Bovine Embryo.The result shows that these four pairs of primers all have highly specialty and stability.The nested PCR need only 10pg bovine genomic DNA to determine the sex of embryo,but the normal PCR need 50pg,so the nested PCR is more suitable for sex determination of bovine embryo. |
| Keywords: | Bovine Embryo Y chromosome repeated sequence Sex determination PCR |
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