| 一株植物乳杆菌的分离鉴定及特性研究 |
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| 引用本文： | 辛国芹,辛国民,徐海燕,武香玉,张志焱,谢全喜.一株植物乳杆菌的分离鉴定及特性研究[J].江西农业学报,2012(8):77-80. |
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| 作者姓名： | 辛国芹 辛国民 徐海燕 武香玉 张志焱 谢全喜 |
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| 作者单位： | 山东宝来利来生物工程股份有限公司;山东省安丘市畜牧局 |
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| 摘 要： | 从泡菜中富集培养分离到12株乳酸菌,经测定发酵液pH值筛选到一株产酸性能较好的菌株PS-9,结合菌落形态特征、生理生化特性及菌株特异性序列分析等手段,确定其分类归属,并对其特性进行了研究。结果表明PS-9为植物乳杆菌(Lactobacillus plantarum),菌株PS-9在pH值2.0环境中的存活率为85.2%,0.4%胆盐浓度环境中存活率为37.2%,对鸡大肠杆菌O78和金黄色葡萄球菌C56011的抑菌圈直径均超过18 mm。
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| 关 键 词： | 分离鉴定 生物学特性 系统发育分析 植物乳杆菌 |
Isolation and Identification of A Strain of Lactobacillus plantarum and Its Characters |
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| XIN Guo-qin,XIN Guo-min,XU Hai-yan,WU Xiang-yu,ZHANG Zhi-yan,XIE Quan-xi.Isolation and Identification of A Strain of Lactobacillus plantarum and Its Characters[J].Acta Agriculturae Jiangxi,2012(8):77-80. |
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| Authors: | XIN Guo-qin XIN Guo-min XU Hai-yan WU Xiang-yu ZHANG Zhi-yan XIE Quan-xi |
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| Institution: | 1(1.Shandong Baolai-leelai Bioengineering Limited Liability Company,Tai’an 271000,China; 2.Anqiu Bureau of Animal Husbandry in Shandong Province,Anqiu 262114,China) |
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| Abstract: | Twelve strains of lactic acid bacteria were isolated from pickles by the enrichment culture method.A strain PS-9 with better acid-forming performance was screened out through the determination of pH-value of the fermentation liquor.It was identified as Lactobacillus plantarum according to its morphology,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The results showed that PS-9 had distinct resistance to acid and bile salt,and its survival rate was 85.2% and 37.2% respectively when it was inoculated in the environment of pH 2.0 and bile salt concentration 0.4%.The inhibitory zone diameters of this strain to avian Escherichia coli O78 and Staphylococcal sp.C56011 were both more than 18 mm. |
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| Keywords: | Isolation and identification Biological characteristics Phylogenetic analysis Lactobacillus plantarum |
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