An investigation on the bacterial flora of Agriotes lineatus (Coleoptera: Elateridae) and pathogenicity of the flora members |
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| Institution: | 1. Karadeniz Technical University, Faculty of Science, Department of Biology, 61080 Trabzon, Turkey;2. Rize University, Faculty of Arts and Sciences, Department of Biology, 53100 Rize, Turkey;1. QIMR Berghofer Centre for Immunotherapy and Vaccine Development and Tumor Immunology Laboratory, Brisbane, Australia;2. Human Immunity Laboratory, Brisbane, Australia;3. Immunology in Cancer and Infection Laboratory, Department of Immunology, QIMR Berghofer Medical Research Institute, Brisbane, Australia;4. Department of Nephrology, Princess Alexandra Hospital, Woolloongabba, Australia;5. Queensland Lung Transplant Service, The Prince Charles Hospital, Brisbane, Australia;6. School of Medicine, The University of Queensland, Brisbane, Australia;1. State Key Laboratory of Integrated Management of Pests and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China;2. Key Laboratory of Mountain Ecological Restoration and Bio-resource Utilization & Ecological Restoration Biodiversity Conservation, Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;3. Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;4. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China;1. Bioprojet-Biotech, 4 rue du Chesnay Beauregard, BP 96205, 35762 Saint-Grégoire, France;2. Heinrich Heine University Düsseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany;1. Department of Fish and Wildlife Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060, United States;2. Southeast Fisheries Science Center, NOAA National Marine Fisheries Service, Miami, FL, 33149, United States;1. Laboratory of Agroforestry Pathology, Department of Agronomy, ETSIAM, University of Cordoba, Campus of Rabanales, Edificio C4, 14071 Córdoba, Spain;2. Laboratory of Agricultural Entomology, Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, Campus de Rabanales, Edificio C4, 14071 Cordoba, Spain;3. Colombian Agricultural Research Corporation (CORPOICA), Research Center (Nataima), Km 9, Espinal-Ibague, Tolima, Colombia |
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| Abstract: | The wireworm Agriotes lineatus (L.) (Coleoptera: Elateridae) is a serious agricultural pest of various vegetables and fruits throughout the world. To find an effective and safe biological control agent against this pest, we investigated the bacterial flora of A. lineatus. Nineteen different bacterial strains were isolated and identified as Paenibacillus sp. (Ag1), Cellulomonas sp. (Ag2), Bacillus subtilis (Ag3), Staphylococcus sp. (Ag4), Enterococcus mundtii (Ag5), Staphylococcus sp. (Ag6), Sphingobacterium sp. (Ag7), Staphylococcus pasteuri (Ag8), Arthrobacter gandensis (Ag9), Bacillus sp. (Ag10), Chryseobacterium sp. (Ag11), Streptomyces sp. (Ag12), Oerskovia turbata (Ag13), Bacillus thuringiensis (Ag14), Pseudomonas fluorescens (Ag15), Oerskovia jenensis (Ag16), Arthrobacter gandavensis (Ag17), B. thuringiensis (Ag18), and Pseudomonas plecoglossicida (Ag19) based on conventional and molecular tests. A. gandavensis and P. plecoglossicida were isolated for the first time from any insect. The insecticidal effects of these 19 bacterial isolates and the additional 11 isolates belonging to Bacillus genus isolated from different hosts were tested on third instar larvae of A. lineatus. Ag17 (A. gandavensis), Ag18 (B. thuringiensis), and Ag19 (P. plecoglossicida) from the bacterial flora of A. lineatus, and two Bacillus isolates (Bacillus circulans Ar1 from Anoplus roboris and B. thuringiensis subsp. kurstaki BnBt from Balanicus nucum) showed 100% mortality 10 days after treatment. Our results indicate that the bacterial isolates tested in this study may be considered as a possible microbial control agent against A. lineatus. |
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