Species specific primers for identification of Alternaria solani,in combination with analysis of the F129L substitution associates with loss of sensitivity toward strobilurins |
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| Institution: | 1. Department of Botany, Udai Pratap Autonomous Post Graduate Institution, Bhojubir, Varanasi, U.P. 221 002, India;2. Division of Crop Improvements, ICAR – Indian Institute of Vegetable Research, P. B. No. 5001, P.O. BHU, Varanasi, U.P. 221 005, India;3. Division of Crop Protection, ICAR – Indian Institute of Vegetable Research, P. B. No. 5001, P.O. BHU, Varanasi, U.P. 221 005, India;1. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;2. Wageningen University and Research Centre (WUR), Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands;3. Utrecht University, Department of Biology, Microbiology, Padualaan 8, 3584 CH Utrecht, The Netherlands;1. College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, PR China;2. College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, PR China;1. Department of Bioresource and Food Science, Konkuk University, 120 Neundong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea;2. Department of Plant Science, Bharathidasan University, Palkalaiperur, Tiruchirappalli 620024, Tamil Nadu, India;3. Department of Biochemistry and Molecular Biophysics, Kansas State University, 238 Burt Hall, Manhattan, KS 66506, USA |
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| Abstract: | A species specific forward primer was designed in order to identify the phytopathogenic fungus Alternaria solani, which causes early blight on potato. The reverse primer came from another survey of the American population of A. solani. The combination of these two primers can be used both as molecular identification of A. solani as well as sending the PCR product for sequencing in search for the F129L substitution associated with loss of sensitivity toward fungicides based on strobilurins. The diagnostic method has been successfully used when identifying the causal agent of lesions on potato leaflets during large field assays. |
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