玉露的离体培养与快繁研究

【目的】为快速繁殖玉露优良品种,筛选出玉露最佳繁殖方法。【方法】以玉露叶片为外植体,采用不同消毒时间、不同浓度的培养基进行离体培养。【结果】最佳启动培养基为MS+6-BA 3.0 mg/L+NAA 0.6mg/L,愈伤组织诱导培养基MS+6-BA 0.5mg/L+NAA 0.01mg/L,愈伤组织诱导率达82.3%,分化培养基同诱导培养基,丛芽分化率达44.3%,生根培养基为1/2MS+NAA 1 mg/L+AC 0.5g/L,生根率达98.5%,经炼苗成活率高达91%以上。【结论】该研究为玉露离体培养技术研究提供技术支撑。

Invitro culture and rapid propagation of Haworthia cooperi var.pilfera

Objective]Haworthia cooperi var.Pilfera belong to Liliaceae haworthia plant.To rapidly product excellent varieties of pilfera,leaves were used as explants to establish tissue culture system.Methods]Compared different disinfecting time and different concentrations of hormones in different culture media to screen the best tissue culture methods.Results]The best starting culture medium was MS with 6-BA (3.0 mg/L) and NAA (0.6 mg/L),and the best callus induction medium was MS with 6-BA (0.5 mg/L) and NAA (0.01 mg/L).The results revealed that callus induction rate was 82.3 ％,the differentiation medium was same with induction medium,and the bud differentiation rate was 44.3％.The best rooting medium was 1/2 MS with NAA (1 mg/L)and AC (0.5 g/L),the rooting rate was 98.5％,and the seedling survival rate was more than 91％.Conclusion]This study provides technical support for Haworthia cooperi var.pilfera in vitro culture technology research.