禁食禁水对新疆伊犁马生化指标及应激相关激素的影响

为探究禁食禁水双重应激对马匹机体的影响,试验选取8匹成年伊犁骟马,采用不完全拉丁方试验设计,分为禁食禁水组(禁食禁水49 h)和正常饲喂组(对照组),在试验过程中监测马匹基本生命体征、血液生化指标及应激相关激素含量的变化。结果表明,禁食禁水组心率在12~48 h均显著低于对照组(P< 0.05),呼吸频率在48和49 h显著低于对照组(P< 0.05),血糖浓度在18、24和36 h均显著低于对照组(P< 0.05),血浆皮质醇水平在18、30和36 h均显著高于对照组(P< 0.05),血浆抗利尿激素水平在48和49 h均显著高于对照组(P< 0.05),其他指标未见显著变化(P> 0.05)。由此可知,在禁食禁水双重应激情况下,马匹通过降低心血管活动、减少机体能量消耗、提高应激能力来维持基本生命活动。

Effect of Fasting and Water Deprivation on Biochemical Indexes and Stress-related Hormones in Yili Horses

The aim of the present study was to explore the influence of both fasting and water deprivation stress on horses.Eight adult Yili gelding were used with a incomplete Latin square design.The animals were divided into fasting and water deprived for 49 h group (feed and water deprivation group, FW group) and normal feeding group (control group).The changes of basic vital signs, blood biochemistry indexes and stress-related hormones indicators were monitored during experiment from horses.The results showed that heart rate of FW group at 12 to 48 h were significantly lower than the control group (P< 0.05), breathing rate at 48 and 49 h were significantly lower than the control group (P< 0.05), blood glucose concentration at 18, 24 and 36 h were significantly lower than the control group (P< 0.05), plasma cortisol level at 18, 30 and 36 h were significantly higher than the control group (P< 0.05), plasma antidiuretic hormone level at 48 and 49 h were significantly higher than the control group (P< 0.05).There were no significant differences in other parameters between two groups (P> 0.05).The results suggested that horses could maintain basic life activities by reducing cardiovascular activity and body energy consumption, and improving the stress ability in the condition of fasting and water deprivation.