鸭甲肝病毒GX株全基因组序列测定及VP1蛋白生物信息学分析

为探讨鸭甲肝病毒(DHAV)GX株基因分型特点及主要衣壳蛋白(VP1)的生物学特性,本试验对其进行全基因组序列测定,并应用分子生物学软件将DHAV GX株与DHAV 3个血清型参考毒株进行序列比对分析。结果显示,其基因组全长7 800 bp,由5'和3'非编码区(UTR)和一个大开放阅读框(ORF)组成。其中,5'UTR和3'UTR的长度分别为652和369 bp;ORF长度为6 756 bp,编码2 251个氨基酸长的多聚蛋白,其编码产物至少有12个(VP0/VP3/VP1/2A1/2A2/2A3/2B/2C/3A/3B/3C/3D);在分类地位上DHAV GX株属于DHAV-3,其与DHAV-3参考株核苷酸、氨基酸同源性最高;与DHAV-3 FS株亲缘关系最近,在同一较小分支上。DHAV GX株结构蛋白VP1以第195—201、211—221位氨基酸区段为B细胞优势表位的可能性较大。提示,VP1基因可作为研制DHAV基因工程疫苗的优势候选基因。

Determination of the Complete Genome of DHAV GX Strain and Bioinformatics Analysis of VP1 Protein

In order to investigate the genotypic characteristics and biological characteristics of major capsid protein (VP1) of duck hepatitis A virus (DHAV) GX strain, the whole genome of GX strain was sequenced and compared with 3 serotypes reference strains of DHAV by the molecular biology software.The results showed that the full-length genome of DHAV GX strain was 7 800 bp, including 5'UTR (652 bp), 3'UTR (369 bp) and ORF (6 756 bp) encoding 2 251 aa and its coding products were 12 (VP0/VP3/VP1/2A1/2A2/2A3/2B/2C/3A/3B/3C/3D) at least.Sequence analysis showed that DHAV GX strain could be ranked DHAV 3 style.This strain shared the highest homology with DHAV-3 reference strains; Furthermore, GX strain and DHAV-3 FS strain were in the same cluster, might come from the same ancestor.Amino acid segments locating in 195 to 201 and 211 to 221 aa could be larvaceous dominant B-cell linear epitopes.It suggested that VP1 gene could be used as a candidate gene for the development of genetic engineering vaccine of DHAV.