|
|||||
|
|
| 不同浓度神经肽P物质刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响 | |
| 引用本文： | 王亚娟,高峰,刘迎春,周欢敏.不同浓度神经肽P物质刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响[J].中国畜牧兽医,2015,42(9):2298-2302. |
| 作者姓名： | 王亚娟 高峰 刘迎春 周欢敏 |
| 作者单位： | 1. 内蒙古农业大学生命科学学院, 呼和浩特 010018; 2. 内蒙古农业大学动物科学学院, 呼和浩特 010018; 3. 内蒙古自治区生物制造重点实验室, 呼和浩特 010018 |
| 基金项目： | 国家自然科学基金(30901016、31160445) |
| 摘 要： | 本研究旨在探讨不同浓度的神经肽P物质(SP)刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响。本研究分别采用0(对照组)、1×10-6、1×10-7、1×10-8、1×10-9 mol/L 5个浓度梯度的SP对内蒙古白绒山羊皮肤干细胞进行刺激处理,并分别于第0、7、14及21天采用实时荧光定量PCR方法鉴定BMP2和BMP4基因的表达量。结果显示,1×10-6和1×10-8 mol/L的SP促进BMP2、BMP4的表达;在7和14 d时,SP 1×10-8 mol/L组BMP2的表达量显著高于SP 1×10-6 mol/L组(P<0.05),SP 1×10-6 mol/L组BMP4的表达量与SP 1×10-8 mol/L组差异不显著(P>0.05);在21 d时,SP 1×10-8 mol/L、SP 1×10-6 mol/L组BMP2、BMP4的表达量都明显降低,其中SP 1×10-8 mol/L组BMP2的表达量显著高于1×10-6 mol/L组(P<0.05),SP 1×10-6 mol/L组BMP4的表达量与SP 1×10-8 mol/L组差异不显著(P>0.05)。结果表明,SP 1×10-6和1×10-8 mol/L不仅对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因的表达量有影响,而且为皮肤干细胞的定向分化提供理论基础。 |
| 关 键 词： | 皮肤干细胞 神经肽P物质 BMP 表达 |
| 收稿时间： | 2015-03-12 |
Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat |
|
| WANG Ya-juan,GAO Feng,LIU Ying-chun,ZHOU Huan-min.Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat[J].China Animal Husbandry & Veterinary Medicine,2015,42(9):2298-2302. | |
| Authors: | WANG Ya-juan GAO Feng LIU Ying-chun ZHOU Huan-min |
| Institution: | 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. Key Laboratory of Inner Mongolia Autonomous Region Biological Manufacturing, Hohhot 010018, China |
| Abstract: | This study was aimed to explore the expression of BMP2 and BMP4 genes in skin stem cells of Mongolia Cashmere goat with the influence of different concentrations of neuropeptide P (SP).We set five different SP concentrations (0, 1×10-6, 1×10-7, 1×10-8 and 1×10-9 mol/L) to stimulate the skin stem cells, then identified the expression quantities of BMP2 and BMP4 at days of 0, 7, 14 and 21 by Real-time quantitative PCR with the purpose of determining the optimum SP concentration for the differentiation of skin stem cells.The results showed that SP with the concentrations of 1×10-6 and 1 ×10-8 mol/L promoted the expression of BMP2 and BMP4;The expression of BMP2 at 7 and 14 d in SP 1×10-8 mol/L group was significantly higher than that of the SP 1×10-6 mol/L group (P<0.05);The expression of BMP4 in SP 1×10-6 mol/L group had no significant difference with the SP 1×10-8 mol/L group (P>0.05);At 21 d, the expression of BMP2 and BMP4 significantly decreased in SP 1×10-8 and the SP 1×10-6 mol/L groups, and the expression of BMP2 in the SP 1×10-8 mol/L group was significantly higher than that of the 1×10-6 mol/L group (P<0.05), while the expression of BMP4 in the SP 1×10-6 mol/L group had no significant difference with that of SP 1×10-8 mol/L group (P>0.05).The results indicated that the concentrations of 1×10-6 and 1×10-8 mol/L not only might affect the expression of BMP2 and BMP4 of skin stem cells, but also provided a theoretical basis for the directional differentiation of skin stem cells. |
| Keywords: | skin stem cells neuropeptide P BMP expression |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 | |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 | |