海南五指山猪Myf5与MyoD基因多态性分析

为研究海南五指山猪Myf5和MyoD基因的多态性分布情况,本试验通过PCR-RFLP方法对80头海南五指山猪进行了Myf5和MyoD基因型检测,并运用生物信息学方法对这两个基因的序列进行分析.结果发现,在Myf5基因第1外显子的HhaⅠ-RFLP位点上,五指山猪以CC和CG基因型为主;在Myf5基因第1外显子的Hsp92Ⅱ-RFLP位点上,五指山猪以AA基因型为主;在MyoD基因第1内含子的DdeⅠ-RFLP位点上,等位基因A在五指山猪中是优势等位基因.此外,Myf5和MyoD基因启动子区域和第1外显子处存在CpG岛.本研究得到海南五指山猪Myf5和MyoD基因的序列信息,为下一步五指山猪Myf5和MyoD基因的功能研究奠定基础.

Polymorphism Analysis of Myf5 and MyoD Genes in Hainan Wuzhishan Pig

This study was aimed to investigate the distribution of genetic polymorphisms of Myf5 and MyoD genes in Hainan Wuzhishan pig.PCR-RFLP method was used to detect the Myf5 and MyoD of 80 Wushishan pigs.Some characters of Myf5 and MyoD genes were analyzed by bioinformatics tools.The main results were as follows at the HhaⅠ-RFLP locus of exon 1 in Myf5 gene,CC and CG genetypes were major in Wuzhishan pig.At the Hsp92Ⅱ-RFLP locus of exon 1 in Myf5 gene,AA genetype was major in Wuzhishan pig.At the DdeⅠ-RFLP locus of intron 1 in MyoD gene,allele A was predominant in Wuzhishan pig.In addition,bioinformatics analysis revealed that Myf5 and MyoD genes promoter and exon 1 region contained CpG islands.In this study,we obtained the sequences of Myf5 and MyoD genes which laid a foundation for further studying their functions in Hainan Wuzhishan pig.