Pax7基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律研究

本试验旨在探究Pax7(paired box 7)基因在猪不同组织中的表达特征及其在背最长肌中的发育性表达规律.采用实时荧光定量PCR(qRT-PCR)技术和Western blotting技术检测了Pax7基因在1日龄马身猪心脏、肝脏、脾脏、肺脏、肾脏、胃、小肠、下丘脑、小脑、背最长肌、腰大肌、股二头肌12种组织中的mRNA和蛋白质表达谱,以及马身猪和大白猪从出生1日龄到180日龄7个发育阶段(1、30、60、90、120、150、180日龄)背最长肌中的发育性表达规律.结果表明,Pax7基因mRNA在背最长肌、腰大肌、股二头肌、下丘脑和小脑组织中表达,而Pax7蛋白仅在背最长肌、腰大肌和股二头肌中表达.背最长肌中Pax7基因mRNA和蛋白质的发育性表达趋势在马身猪和大白猪中基本一致.在mRNA水平上,30日龄的表达量最高,极显著高于其他日龄(P <0.01);1日龄的表达量次之;其他日龄维持低表达的稳定状态.Pax7蛋白表达量在1日龄时最高,极显著高于其他日龄(P <0.01);30日龄次之;其他日龄维持低表达的稳定状态.从出生1日龄到180日龄,马身猪背最长肌中Pax7蛋白的表达量均显著或极显著地高于大白猪(P <0.05;P <0.01).Pax7 基因的表达与组织、日龄及品种的遗传背景有关.

Study on the Expression Profile of Pax7 Gene and its Developmental Expression in Longissimus Dorsi of Pigs

The aim of this study was to investigate the expression profile of Pax7 (paired box 7) gene in different tissues and its developmental expression in longissimus dorsi of pigs.The expression profiles of Pax7 gene at the levels of mRNA and protein in 12 different tissues including heart,liver,spleen,lung,kidney,stomach,small intestine,hypothalamus,cerebellum,longissimus dorsi,biceeps femoris,psoas major of Mashen pigs at birth and the developmental expression patterns in longissimus dorsi at seven stages (1,30,60,90,120,150,and 180-day old) of Mashen and Large White pigs were studied by quantitative Real-time PCR and Western blotting.The results showed that the Pax7 mRNA was expressed in longissimus dorsi,biceeps femoris,psoas major,hypothalamus,and cerebellum,wherease the Pax7 protein was only expressed in skeletal muscles of longissimus dorsi,biceeps femoris,and psoas major.The developmental expression patterns of Pax7 mRNA and protein in Mashen pig was basically in accordance with those in Large White pig.The mRNA expression amount of Pax7 gene in longissimus dorsi was the greatest at 30-day old (P <0.01),followed by the expression at 1-day old,and kept lower stable levels at other stages both in Mashen and Large White pigs.At the stages of 30-day,90-day,and 180-day old,Pax7 mRNA expression amounts in Mashen pig were significantly higher than those in Large White pig (P <0.01).The Pax7 protein expression at 1-day old was the greatest (P <0.01),followed by it at 30-day old,which were significantly greater than those at other stages,at which Pax7 expression was in lower stable levels.The Pax7 protein expression from one-day old to 180-day old in Mashen pig was significantly greater than those in Large White pig (P <0.05;P <0.01).The expression of Pax7 gene could associated with tissue,age,and background of pig breed.