鹅细小病毒侵染雏鹅组织器官的氧化/脱氢酶及同工酶研究

酶是催化代谢途径的高效活性基因产物,应激环境和遗传基础的不同导致同工酶结构或活性差异,使不同组织和发育阶段的酶种类和活性表现特异性变化。采用生化分析试验技术和PAGE分析鹅细小病毒(GPV)感染雏鹅氧化/脱氢酶(LDH、ADH、POD、CAT)的活性及同工酶特征变异,结果表明,GPV感染雏鹅的脑、心脏、脾脏组织新出现1条POD同工酶谱带,肺脏组织消失1条同工酶谱带;GPV感染雏鹅的肝脏、脾脏组织分别缺失慢区3条和1条CAT同工酶谱带;GPV感染雏鹅组织器官的LDH和ADH同工酶仅显示1~2个慢区带,肺脏缺失1条慢区ADH同工酶谱带,ADH活性降低了13.61%~61.08%,心脏增高1.2倍;POD、CAT、LDH活性分别增强1.2~6、1.5~3.5、2~2.5倍,而脑、肺脏的CAT活性降低了40.29%和94.68%,心脏、肺脏的LDH活性降低了75.93%和91.81%。提示氧化/脱氢酶产生的广泛变异是GPV感染应激与宿主氧化/脱氢酶基因表达的互作效应,其酶的谱型、活性等生化特征变异,直接或间接调控代谢途径、影响生理机能及病理性能,敏感的氧化/脱氢酶是研究病毒基因型与宿主遗传易感性互作病理学机制的有效标记物。

Study on the Oxidase/Dehydrogenase Enzymes and Isozyme from Tissues and Organs of Goslings Infected by Goose Parvovirus

The enzyme was efficient active gene product of catalyzing metabolic pathway.Stress environment and genetic basis led the differences of isozyme structure or activity, which specific changes of the kinds and activities of enzyme were showed in different tissues and developmental stages.Here, lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), peroxydase (POD) and catalase (CAT) were respectively extracted from brain, heart, liver, lung and spleen tissues and organs of goslings infected by goose parvovirus (GPV) and control group.The results of PAGE and biochemical analysis showed that there respectively were 3 and 1 slow-zone CAT isozyme bands deletion in liver and spleen of goslings infected by GPV.There respectively were 1 new enzyme band of POD isozyme in the brain, heart and spleen of goslings infected by GPV, but 1 enzyme band of POD isozyme deletion in the lung of goslings infected by GPV.It only showed 1 to 2 slow-zone LDH and ADH isozyme bands in organ and tissue of goslings infected by GPV, and deleted 1 slow-zone ADH isozyme band in the lung of goslings infected by GPV.The activity of ADH from goslings infected by GPV were reduced 13.61% to 61.08%, but increased 1.2 times in the heart of goslings infected by GPV.The activity of POD, CAT and LDH from goslings infected by GPV were higher 1.2 to 6, 1.5 to 3.5, 2 to 2.5 times than that of control group, respectively.The activity of CAT reduced 40.29% and 94.68% in the brain and lung of goslings infected by GPV.The activity of LDH reduced 75.93% and 91.81% in liver and lung of goslings infected by GPV.Those results indicated that the interaction of GPV stress and host oxidase/dehydrogenase enzyme gene expression resulted in the biochemical characteristics variation of activity and isozyme patterns of oxidase/dehydrogenase enzyme, and directly or indirectly affected metabolic approach and physiological pathological function on goslings infected by GPV.Therefore, sensitive oxidase/dehydrogenase was the effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.