| 猪流行性腹泻病毒SHpd/2012株S1和S2基因片段的真核表达 |
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| 引用本文： | 赵玉婷,谢洋洋,周书亭,丁思嘉,蔡雨豪,周艳君,杨志彪. 猪流行性腹泻病毒SHpd/2012株S1和S2基因片段的真核表达[J]. 上海交通大学学报(农业科学版), 2017, 0(5): 69-73 |
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| 作者姓名： | 赵玉婷 谢洋洋 周书亭 丁思嘉 蔡雨豪 周艳君 杨志彪 |
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| 作者单位： | 上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海海洋大学 水产与生命学院, 上海 201306,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,中国农业科学院 上海兽医研究所,上海 200240,上海交通大学 农业与生物学院,上海 200240 |
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| 基金项目： | 国家重点研发计划(2016YFD0500100);国家自然科学基金(31472211);上海市自然基金(14ZR1421400) |
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| 摘 要： | 为探究猪流行性腹泻病毒(PEDV)的新毒株SHpd/2012在感染细胞时与细胞的相互作用机制,根据已得到的序列设计引物,克隆了S1和S2基因片段,构建了pCAGGS-S1和pCAGGS-S2真核表达质粒。间接免疫荧光和Western-blot实验表明,转染后S1和S2都能在Vero细胞内表达,且转染48h后的表达量较高;其中S1蛋白分子量约为90kDa,S2蛋白分子量约为60kDa。流行毒株SHpd/2012的S1与S2两肽段的成功表达,为之后进一步研究PEDV与Vero细胞的相互作用奠定了基础。
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| 关 键 词： | 猪流行性腹泻病毒 S基因 真核表达 |
| 收稿时间： | 2016-10-24 |
Eukaryotic Expression of S1 and S2 Gene Fragments from SHpd/2012 Strain of Porcine Epidemic Diarrhea Virus |
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| ZHAO Yu-ting,XIE Yang-yang,DING Si-ji,ZHOU Shu-ting,CAI Yu-hao,ZHOU Yan-jun and YANG Zhi-biao. Eukaryotic Expression of S1 and S2 Gene Fragments from SHpd/2012 Strain of Porcine Epidemic Diarrhea Virus[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2017, 0(5): 69-73 |
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| Authors: | ZHAO Yu-ting XIE Yang-yang DING Si-ji ZHOU Shu-ting CAI Yu-hao ZHOU Yan-jun YANG Zhi-biao |
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| Affiliation: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai,200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai,200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai,200240,China,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai,200240,China,Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai,200240,China |
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| Abstract: | S1 and S2,fragments of S gene in SHpd/2012 strain of porcine epidemic diarrhea virus (PEDV),were cloned and their eukaryotic expression plasmids,namely pCAGGS-S1 and pCAGGS-S2,were constructed to investigate the mechanism of the interaction between SHpd/2012 strain of porcine epidemic diarrhea virus and Vero cells.Indirect immunofluorescence and Western-blot assay results showed that S1 and S2 could be expressed well in Vero cells and the amount of peptides were high 48 h after transfection.The molecular weights of S1 and S2 peptide were 90 and 60 kDa,respectively.The successful expression of the S1 and S2 proteins of the new strain SHpd/2012 laid the foundation for the further study of the interaction between PEDV and Vero cells. |
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| Keywords: | porcine epidemic diarrhea virus S gene eukaryotic expression |
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