Effect of skeletal muscle decorin on collagen fibrillogenesis in vitro |
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Authors: | Takanori NISHIMURA Ayaka TANEICHI Jun-ichi WAKAMATSU Akihito HATTORI |
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Institution: | Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo-shi, Japan |
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Abstract: | The effect of skeletal muscle decorin on collagen fibrillogenesis was investigated, in order to provide background for understanding the functions of decorin in skeletal muscle. The self‐assembly of type I and III collagen with the addition of decorin or the core protein of decorin from bovine neonatal skeletal muscle was monitored using a spectrophotmeter. Time course changes in the absorbance of collagen solutions showed typical sigmoidal curves composed of three phases. The time of the initial phase was not different between the collagen solution with decorin and that without decorin. The increase rate of the absorbance in the second phase decreased with concentration of decorin added in collagen solutions. Similar effects on fibrillogenesis of type I and III collagens were observed when the core protein of decorin was added in collagen solutions. These results suggest that regulation of collagen fibrillogenesis by decorin depends on its core protein. The networks of reconstructed collagen fibrils with decorin were looser than those without decorin. Bovine skeletal muscle decorin could participate in the regulation of collagen fibrillogenesis and in the arrangement of collagen fibrils in the intramuscular connective tissue. |
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Keywords: | collagen connective tissue decorin fibrillogenesis muscle |
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