Interspecific hybrid of Helianthus annuus × H. simulans: Characterization and utilization in improvement of cultivated sunflower (H. annuus L.) |
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Authors: | AJ Prabakaran M Sujatha |
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Institution: | (1) Crop Improvement Section, Directorate of Oilseeds Research, Rajendranagar, Hyderabad, 500 030, India |
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Abstract: | The first success at interspecific hybridization between cultivated sunflower(H. annuus) and a diploid perennial species, H. simulans is reported. The F1s from both direct and reciprocal directions exhibited dominance of the wild species phenotype and were pollen sterile. Meiosis
was irregular in the F1 hybrids and both univalents and multivalents were observed. Multiplication of the interspecific hybrids was achieved through
in vitro culture of nodal sections on Murashige & Skoog medium supplemented with 0.5 mg l-1 benzyladenine. Fertility of the interspecific hybrids was improved by subjecting the in vitro proliferating shoots to 0.001% colchicine incorporated in the shoot multiplication medium. The amphiploids serve as fertile
bridges and facilitate interspecific gene transfer through conventional breeding.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | chromosome doubling Helianthus annuus H simulans interspecific hybrid meiosis micropropagation pollen sterility |
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