HCl和DMSO阻断滞育条件下桑蚕卵壳电镜观察

【目的】探讨桑蚕Bombyx mori滞育阻断机理,比较HCl、二甲基亚砜(DMSO)法阻断蚕卵滞育后卵壳表面发生的一系列变化。【方法】用HCl和DMSO分别在产后20和12 h处理蚕卵,利用扫描电镜观察卵壳表面及气孔在处理后不同时间点的变化。【结果】HCl和DMSO处理均能成功地阻断蚕卵滞育的发生,蚕卵平均孵化率分别为93.37%和87.81%。扫描电镜观察HCl和DMSO处理的卵壳表面蚀刻效果明显,均出现了“脂质”脱落物;并且处理后蚕卵的气孔有逐步增大的趋势,与DMSO处理相比较,HCl处理获得的现象更为明显。【结论】卵壳表面变化可能与桑蚕滞育的阻断有关。

SEM observation on silkworm eggshell under HCl or dimethyl sulfoxide (DMSO) treatment for preventing diapause

Objective] To investigate the diapause prevention mechanism of silkworm ( Bombyx mori ) , and compare a series of changes of eggshell surface after diapause prevention by HCl or DMSO treatment .Method]The eggs were treated with HCl or DMSO at 20 and 12 h after oviposition respectively .Scan-ning electron microscope ( SEM ) was used to observe the changes of eggshell surface and aeropyle at different time points after treatment .Result]Both HCl and DMSO treatments successfully prevented the occurrence of egg diapause , and the average hatching rates were 93.37% and 87.81% respectively . SEM observation showed clear etching effect and “lipid” shed on eggshell surface , and the aeropyle diameter increased gradually under both treatments .The above observations were more obvious for the HCl treatment compared with the DMSO treatment .Conclusion]Eggshell surface changes may be related to the diapause prevention of silkworm .