一个新的大豆细胞质黄化突变体的初步研究

用一个大豆叶片黄化突变体与6个带有不同标记基因的基因型杂交配制了8个杂交组合(包括两组正反交组合)。根据杂交后代的表现对该突变体进行了遗传分析并测定了亲本和F_1植株的叶绿素含量。结果表明该突变体的叶绿素缺失性状呈母体遗传。当以黄化突变体为母本时,杂交F_1和F_2单株都表现为黄化,当用正常的非黄化基因型作为母本时,所有的F_1和F_2植株都不黄化。在自然光照条件下,突变体的叶绿素a、叶绿素b和总叶绿素含量约是正常基因型的47.4、40.4和43.7％。突变体新生叶片的叶绿素含量很低,随着叶片的发育成熟,叶绿素含量逐渐接近正常基因型。根据大豆遗传委员会的有关规定和惯例,建议将该突变体定名为Cyt—Y_4。

A PRELIMINARY STUDY ON A CYTOPLASMICALLY INHERITED YELLOW FOLIAR MUTANT IN SOYBEANS

Eight crosses were made with a yellow foliar mutant and 6 normal pigment genotypes including 2 set of reciprocal crosses. Genetic analysis were made based on the performance of F1 and F2 populations. The contents of chlorophyll of parents and F1 plants were detemined. The results indicated that the chlorosis character of the mutant was maternally inherited. When the yellow foliar mutant was used as the female parents, all the plants in all F1 and F2 populations were chlorosis. While if the pigment normal genotypes were used as the female parents, all the plants in F1 and F2 populations were normal. The contents of chlorophyll a, b and a+b were about 47. 4, 40. 4 and 43. 7% of that from the normal genotypes under natural photosynthetic photon flux densities (PPFD). The content of chlorophyll of the young leaves from the mutant plants were extremely low. It gradually become normal with the development of the leaves. According to the rules and regulations established by the Soybean Genetic Committee, the mutant genotype was designated as cyt-Y4.