植物激素对珍珠芦荟愈伤组织诱导和芽分化增殖的影响

为珍珠芦荟快繁体系的建立提供理论基础。以珍珠芦荟嫩茎嫩叶为外植体,通过正交试验探讨不同浓度和比例的植物激素对其愈伤组织诱导和芽分化增殖的影响。2,4-D为珍珠芦荟诱导愈伤组织的主要影响因素,浓度为1.5 mg/L时诱导效果最好。珍珠芦荟愈伤组织形成的最佳激素组合为1.5 mg/L 2,4-D+0.6 mg/L 6-BA+0.3 mg/L NAA+0.1 mg/L KT,愈伤组织呈黄绿色松散状,且形成率达100%;芽分化的最佳激素组合为1.2 mg/L 6-BA+0.1 mg/L NAA,适当增加6-BA浓度可提高愈伤组织出芽率。培养基的水分含量对愈伤组织形成及出芽有一定影响。该研究为珍珠芦荟的离体快繁及产业化生产提供了有效途径。

Effects of Plant Hormone on Callus Induction and Bud Differentiation and Proliferation of Aloe aristata Haw

The research aimed to provide theoretical basis for the establishment of the rapid propagation system of Aloe aristata Haw.With tender stems and leaves of A.aristata as explants,the effects of different concentrations and proportions of plant hormone on its callus induction and bud differentiation and proliferation were discussed through orthogonal test.2,4-D was a main influencing factor inducing the callus of A.aristata and the induction effect was best at its concentration of 1.5 mg/L.The optimum hormone combination for the formation of A.aristata callus was 1.5 mg/L 2,4-D 0.6 mg/L 6BA 0.3 mg/L NAA 0.1 mg/L KT and the callus presented flavovirens and loose with the formation rate reaching 100%.The optimum hormone combination for the bud differentiation was 1.2 mg/L 6-BA 0.1 mg/L NAA.And properly adding the concentration of 6-BA could increase the germination rate of callus.The water content of medium had certain effects on callus formation and the germination.This research provided an effective approach for the rapid propagation in vitro and industrialization production of Aloe aristata Haw.