蒲包花种子组织培养的研究

[目的]摸索蒲包花种子组织培养技术,解决蒲包花种子播种繁殖率低的问题。[方法]以蒲包花种子为外植体,采用不同的消毒方式、培养基和基质,研究了蒲包花的组织培养。[结果]经浓度70%酒精消毒30 s,蒲包花种子污染率较低,为18.7%,萌发率高,达62.5%。蒲包花不定芽的最佳培养基为MS+BA1.0 ml/L+IAA0.1 ml/L,增殖系数达5.0,丛生芽多,且生长健壮;蛭石+珍珠岩基质对植株的扦插最好,成活率达到63.0%;不经生根培养的试管苗的扦插成活率较高,可达60.0%,经生根培养的试管苗的扦插成活率为48.0%。[结论]IAA对蒲包花不定芽增殖的作用很明显,蒲包花试管苗可不经生根培养直接扦插。

Study on the Tissue Culture of Calceolaria crenatiflora Seeds

[Objective] The study aimed to grope the tissue culture techniques of Calceolaria crenatiflora seeds so as to solve the problem of low sowing propagation rate of C.crenatiflora.[Method] With C.crenatiflora seeds as explants,the tissue culture of C.crenatiflora was studied with different sterilization methods,media and stroma.[Result] Through sterilizing with 70% alcohol for 30 s,the contamination rate was lower,being 18.7 % and the germination rate was higher,being 62.5 %.The optimum medium for C.crenatiflora adventitious buds was MS BA 1.0 ml/L IAA 0.1 ml/L.The multiplication coefficient was up to 5.0.The plantlets grew vigorously with abundant cluster buds.The stroma of vermiculite perlite was best for the cutting of plantlet,with the survival rate up to 63.0 %.The tube plantlets through no rooting culture had higher cutting survival rate,reaching 60.0 %,and that through rooting culture had cutting survival rate of 48.0 %.[Conclusion] IAA had very obvious effect on the multiplication of C.crenatiflora adventitious buds.The tube plantlets of C.crenatiflora could be made for direct cutting through no rooting culture.