| 水溶性癞葡萄降血糖多肽MC2-1-5的分离纯化 |
| |
| 引用本文： | 袁晓晴,顾小红,汤坚,蒋再良.水溶性癞葡萄降血糖多肽MC2-1-5的分离纯化[J].安徽农业科学,2007,35(31):9819-9821. |
| |
| 作者姓名： | 袁晓晴 顾小红 汤坚 蒋再良 |
| |
| 作者单位： | 江南大学食品科学与技术国家重点实验室,江苏无锡,214122;江苏省无锡市富仁生物科技有限公司,江苏无锡,214252 |
| |
| 基金项目： | 无锡市产学研合作项目(DY050012) |
| |
| 摘 要： | 以四氧嘧啶型糖尿病小鼠血糖浓度为筛选指标,通过超滤、凝胶色谱、半制备型反相高效液相色谱分离纯化得到癞葡萄中起降血糖作用的多肽MC 2-1-5。串联飞行时间质谱仪测得该降血糖多肽相对分子量为3 405.517 4 Da。动物试验结果表明,该肽在以2mg/kg剂量灌胃给予四氧嘧啶型糖尿病小鼠时,小鼠的血糖值在灌胃2和4 h后较相应的初始血糖值降低61.70%和69.18%。
|
| 关 键 词： | 癞葡萄 降糖活性 肽 糖尿病 |
| 文章编号： | 0517-6611（2007）31-09819-03 |
| 修稿时间： | 2007-06-08 |
Separation and Purification of a Water Soluble Hypoglycemic Polypeptide MC2-1-5 from Momordica charantia L. Var. abbreviata Ser |
| |
| YUAN Xiao-qing et al.Separation and Purification of a Water Soluble Hypoglycemic Polypeptide MC2-1-5 from Momordica charantia L. Var. abbreviata Ser[J].Journal of Anhui Agricultural Sciences,2007,35(31):9819-9821. |
| |
| Authors: | YUAN Xiao-qing |
| |
| Institution: | State Key Laboratory of Food Science and Technology, Southern Yangtze University, Wuxi, Jiangsu 214122 |
| |
| Abstract: | With the blood glucose concentration of mice with alloxan-induced diabetes mellitus as screening index,the polypeptide MC2-1-5 with hypoglycemic effect in Momordica charantia L.Var.abbreviata Ser was separated and purified through ultrafiltration,gel filtration chromatography and semi preparative reversed phase liquid chromatography(RPLC).The relative molecular weight of the hypoglycemic polypeptide detected by series TOMFS was 3 405.517 4 Da.The results of animal experiments showed that when the mice with alloxan-induced diabetes mellitus was given the polypeptide dose of 2 mg/kg through stomach filling,their blood glucose values decreased 61.70% and 69.18% in 2 and 4 h after stomach filling than the corresponding initial blood glucose value. |
| |
| Keywords: | Momordica charantia L Var abbreviata Ser Hypoglycemic activity Peptide Diabetes mellitus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
