| 6-BA浓度对安祖花愈伤组织与试管苗生长重现性的影响 |
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| 引用本文： | 杨小玲,杨少辉.6-BA浓度对安祖花愈伤组织与试管苗生长重现性的影响[J].安徽农业科学,2007,35(31):9888-9889,9892. |
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| 作者姓名： | 杨小玲 杨少辉 |
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| 作者单位： | 天津市农业科学院,天津,300192;天津大学农业与生物工程学院,天津,300072 |
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| 基金项目： | 天津市科技计划项目(05YGFNC03000和07ZHTGCG00800) |
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| 摘 要： | 同一块安祖花愈伤组织长期连续继代于含有不同浓度6-BA的4种培养基中,分析每代愈伤组织生长量、试管苗分化率及所分化试管苗大小,结果表明:不同培养批次间不同处理重现性较差。所以,在筛选植物适宜培养基时,应把握不同植物的适宜生长温度,并采用多次连续继代培养的方法,以增加结果的可靠性。
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| 关 键 词： | 6-BA 愈伤组织 试管苗 组培 重现性 |
| 文章编号： | 0517-6611（2007）31-09888-02 |
| 修稿时间： | 2007-06-06 |
Effects of BA Concentration on the Reprcducibility of Callus and Test-tube Seedling of Anthurium andraeanum Lind |
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| YANG Xiao-ling et al.Effects of BA Concentration on the Reprcducibility of Callus and Test-tube Seedling of Anthurium andraeanum Lind[J].Journal of Anhui Agricultural Sciences,2007,35(31):9888-9889,9892. |
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| Authors: | YANG Xiao-ling |
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| Institution: | Tianjin Academy of Agricultural Sciences, Tianjin 300192 |
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| Abstract: | The same callus of Anthurium andraeanum Lind were subcultured in 4 kinds of medium contained different concentrations of 6-BA to analyze the growth amount of callus,the differentiation rate of test-tube seedlings and the size of the differentiated seedlings of every generation.The result showed that the reproducibility of different treatments in different subculture times were worse.Therefore,for screening out the suitable plant medium,the optimum growth temperature of different plants should be mastered and the method of continuous and multi-time subculture should be used in order to improve the reliability of the results. |
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| Keywords: | 6-BA Callus Test-tube seedling Tissue culture Reproducibility |
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