| 野百合鳞茎芽的诱导和增殖的初步研究 |
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| 引用本文： | 莫昭展,符韵林,戚萌.野百合鳞茎芽的诱导和增殖的初步研究[J].安徽农业科学,2007,35(31):9890-9892. |
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| 作者姓名： | 莫昭展 符韵林 戚萌 |
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| 作者单位： | 玉林师范学院,广西玉林,537000;广西大学林学院,广西南宁,530004 |
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| 基金项目： | 广西教育厅科技项目，玉林师范学院青年项目 |
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| 摘 要： | 以野生百合的鳞茎为外植体,研究鳞茎的最佳消毒时间、诱导鳞茎芽的最佳部位、影响鳞茎芽诱导的最佳激素组合以及不同激素对鳞茎芽增殖的影响。结果表明:用75%酒精30 s+0.1%HgCl25 min消毒效果最好。鳞茎芽的诱导能力依次是:内层>中层>外层;基部>中部>上部。MS+NAA 0.5 mg/L+6-BA 1.5 mg/L对鳞茎芽的诱导比较好,MS+6-BA 0.8 mg/L+NAA 0.05 mg/L对鳞茎芽的增殖比较好。
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| 关 键 词： | 野百合 鳞茎芽 诱导 增殖 |
| 文章编号： | 0517-6611（2007）31-09890-03 |
| 修稿时间： | 2007-05-26 |
Primary Study on the Induction and Multiplication of Bulb Bud in Lilium brownii |
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| MO Zhao-zhon et al.Primary Study on the Induction and Multiplication of Bulb Bud in Lilium brownii[J].Journal of Anhui Agricultural Sciences,2007,35(31):9890-9892. |
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| Authors: | MO Zhao-zhon |
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| Institution: | Yulin Normal College, Yulin, Guangxi 537000 |
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| Abstract: | With bulb bud in Lilium brownie as explants,the optimum disinfection time,the optimum part of inducing bulb bud,the optimum hormone combination of affecting the induction of bulb bud and the effects of different hormones on the multiplication of bulb buds were studied.The results showed that disinfecting with 75% alcohol for 30 s and 0.1% HgCl2 for 5 min had the best disinfection effect.The inducing abilities of bulb bud were in turn: the internal layer > the middle layer> the external layer;the basal part>the middle part>the top part.MS NAA 0.5 mg/L 6-BA 1.5 mg/L was better for the induction of bulb bud and MS 6-BA 0.8 mg/L NAA 0.05 mg/L was better for the multiplication of bulb bud. |
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| Keywords: | Lilium brownii Bulb bud Induction Multiplication |
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