| 2～3年生三七花药愈伤组织诱导的比较研究 |
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| 引用本文： | 王定康,孙桂芳,郭志明,兰艳芬,杜丽. 2～3年生三七花药愈伤组织诱导的比较研究[J]. 安徽农业科学, 2007, 35(31): 9915-9916 |
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| 作者姓名： | 王定康 孙桂芳 郭志明 兰艳芬 杜丽 |
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| 作者单位： | 昆明师范高等专科学校生物系,云南昆明,650031;云南省昆明医学院教务处,云南昆明,650032;云南省宣威市农业局,云南宣威,655400 |
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| 基金项目： | 云南省教育厅基础研究计划项目（06y081B）;昆明师范高等专科学校校管科研课题. |
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| 摘 要： | 以盆栽2、3年生三七花药为外植体,在附加不同激素、浓度的MS培养基上进行愈伤组织的诱导试验。结果显示:在MS基本培养基中附加2,4-D 2.0 mg/L+BA 1.5 mg/L+IAA 1.0 mg/L+蔗糖6%+琼脂0.8%的条件下,2年生三七花药愈伤组织的诱导率可达43.0%,三年生三七花药愈伤组织的诱导率可达55.0%。2、3年生三七花药愈伤组织继代培养差别不大。
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| 关 键 词： | 2年生三七 3年生三七 花药培养 愈伤组织 诱导 |
| 文章编号： | 0517-6611（2007）31-09915-02 |
| 修稿时间： | 2007-06-11 |
A Comparative Study on the Inducement of Anther Callus of 2 and 3-Year-Old Panax notoginseng |
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| WANG Ding-kang et al. A Comparative Study on the Inducement of Anther Callus of 2 and 3-Year-Old Panax notoginseng[J]. Journal of Anhui Agricultural Sciences, 2007, 35(31): 9915-9916 |
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| Authors: | WANG Ding-kang et al |
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| Abstract: | With potted 2 and 3-year-old Panax notoginseng anthers as explants,the inducement experiment of callus was conducted on MS medium added different hormones with different concentrations.The results showed that under the condition of adding 2.0 mg/L 2,4-D 1.5 mg/L BA (1.0) mg/L IAA 6%sucrose 0.8% agar to MS basic medium,the inducement rate of the anther callus of 2-year-old P.notoginseng could be up to 43.0%,that of 3-year-old P.notoginseng could be up to 55.0%.Their callus subculture had no big difference. |
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| Keywords: | 2-year-old Panax notoginseng 3-year-old Panax notoginseng Anther culture Callus Inducement |
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