| 萘降解菌NAP2-2的16S rRNA基因克隆和系统发育分析 |
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| 引用本文： | 张春杨,崔艳梅.萘降解菌NAP2-2的16S rRNA基因克隆和系统发育分析[J].安徽农业科学,2007,35(30):9472-9473,9533. |
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| 作者姓名： | 张春杨 崔艳梅 |
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| 作者单位： | 山东理工大学生命科学学院,山东淄博,255091;山东理工大学生命科学学院,山东淄博,255091 |
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| 基金项目： | 山东理工大学博士科研启动基金项目(N04KQ25) |
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| 摘 要： | 筛选到1株萘降解菌NAP2-2,对其进行了表观及16S rRNA基因的系统发育研究。结果表明,NAP2-2能以萘作为唯一碳源和能源生长。16S rRNA基因同源性分析显示,该菌株为短芽孢杆菌属(Brevibacillus)的一个成员。对萘降解菌16S rRNA基因序列的系统发育学分析表明,降解菌主要分布在变形菌门和厚壁菌门。因此对NAP2-2菌株的分析揭示了短芽孢杆菌属新的生物学特性,为以后利用此类细菌处理多环芳烃污染提供了重要依据。
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| 关 键 词： | 萘降解菌 16S rRNA基因 克隆 系统发育分析 |
| 文章编号： | 0517-6611（2007）30-09472-02 |
| 修稿时间： | 2007-06-03 |
Analysis of Cloning and Phylogenetic of 16S rRNA Gene from a Naphthalene Degrading Bacterium NAP2-2 |
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| ZHANG Chun-yang et al.Analysis of Cloning and Phylogenetic of 16S rRNA Gene from a Naphthalene Degrading Bacterium NAP2-2[J].Journal of Anhui Agricultural Sciences,2007,35(30):9472-9473,9533. |
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| Authors: | ZHANG Chun-yang |
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| Institution: | School of Life Sciences;Shandong University of Technology;Zibo;Shandong 255091 |
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| Abstract: | A naphthalene degrading bacterium NAP2-2 was isolated and its 16S rRNA gene phylogeny as well as its apparent character was studied.As a result,strain NAP2-2 grew well with naphthalene as the sole carbon and energy resource.Homological analysis of 16S rRNA gene supported strain NAP2-2 to be a member of Brevibacillus genus.Phylogenetic analysis based on 16S rRNA gene showed that all naphthalene degrading bacteria belong to two phyla: Proteobacteria of G-and Firmicutes of G .Study on strain NAP2-2 revealed a new character(naphthalene degrading) of Brevibacillus and offered an important contribution to the treatment of Poly-aromatic hydrocarbon contaminations with these bacteria. |
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| Keywords: | Naphthalene-degrading bacteria 16S rRNA gene Cloning Phylogenetic analysis |
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