| 甘蔗愈伤组织诱导与植株再生研究 |
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| 引用本文： | 梁巧玲,赵鹂.甘蔗愈伤组织诱导与植株再生研究[J].安徽农业科学,2007,35(32):10246-10247. |
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| 作者姓名： | 梁巧玲 赵鹂 |
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| 作者单位： | 丽水学院化学与生命科学学院,浙江丽水,323000;丽水学院化学与生命科学学院,浙江丽水,323000 |
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| 摘 要： | 以甘蔗粤糖95-168号心叶为材料,分别在添加2,4-D1.5、2.0、2.5、3.0、3.5、4.0、4.5和5.0 mg/L8个处理的MS培养基上培养。试验结果表明,不同浓度的2,4-D对愈伤组织诱导有明显影响,并对愈伤组织分化诱导培养产生影响,其中以2,4-D 2.5 mg/L最佳。幼苗经1/2 MS+NAA1.0 mg/L+AC0.05%培养基诱导生根后成为完整的植株。
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| 关 键 词： | 甘蔗 2 4-D 心叶 愈伤组织 植株再生 |
| 文章编号： | 0517-6611（2007）32-10246-02 |
| 修稿时间： | 2007-06-15 |
Induction of Callus in Saccharum Sinense and the Plant Regeneration |
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| LIANG Qiao-ling et al.Induction of Callus in Saccharum Sinense and the Plant Regeneration[J].Journal of Anhui Agricultural Sciences,2007,35(32):10246-10247. |
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| Authors: | LIANG Qiao-ling |
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| Institution: | College of Chemistry and Life Science, Lishui University, Lishui, Zhejiang 323000 |
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| Abstract: | Central leaves of Saccharum sinense(sugar cane) Strain Yu Tang 95-168 was used as the material,and cultured on MS medium containing 2,4-D at 1.5 mg/L,2.0 mg/L,2.5 mg/L,3.0 mg/L,3.5 mg/L,4.0 mg/L,4.5 mg/L and 5.0 mg/L.The rescults showed that different concentrations of 2,4-D had not only different impacts on the ability of the induction of callus in sugar cane,but also affected the differentiation of injured callus.The 2,4-D concentration of 2.5 mg/L had the most apparent effect.The seedling grew into adult plant in it was induced to put forth root by the medium containing 1/2 MS NAA 1.0 mg/L AC 0.05 %. |
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| Keywords: | Saccharum sinense Central leaf 2 4-D Callus Plant regeneration |
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