The effect of low temperature adversity on male flowers and pollen inLarix olgensis

The electric conductivity of pollen life-force and male flowers ofLarix olgemis on condition of low temperature adversity has been respectively measured by means of fluorescent dying method and conductivity method in 1992 and 1994. The results indicate that: (1) If the same clonal pollen is treated at equal time at different low temperatures, there are significant differences in pollen life-force, the pollen life-force is the largest between O°C and -3°C, in higher or lower temperature range, the pollen life-force will decrease. The measurement of electric conductivity of male flowers proves that the low temperature of below -3°C is the main reason that the cell membrane is damaged and the pollen life-force decreases. (2) The same clonal pollen life-force at the same low temperature will gradually decrease with the increasing of frozen time. Its main reason is that suffocation and losing body fluid result in the damage of cell membrane, the measurement of electric conductivity of male flowers can prove that. (3) If pollen is treated with the same low temperature, the humidity will be the main factor of affecting pollen life-force. (4) There are significant differences inLarix olgensis’s resistance against low temperature in its different growth stage, the male flower’s resistance of near pollen spreading period is low. (5) The resistance is different within clones. (6) The resistance against low temperature ofLarix can be increased by means of chemical treatment.