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何首乌生根培养因子的优选研究
引用本文:陈菊,陈国惠.何首乌生根培养因子的优选研究[J].西南农业大学学报,2006,28(5):756-758.
作者姓名:陈菊  陈国惠
作者单位:西南大学农学与生命科学学院,重庆400716
基金项目:重庆市自然科学基金资助项目(8024)
摘    要:以何首乌嫩茎为外植体,采用正交实验设计进行何首乌生根培养的研究。实验结果表明:影响何首乌生根培养因子的作用大小为培养基〉IBA〉蔗糖〉NAA,诱导何首乌组培苗生根的最佳培养基为:1/2MS+NAA0.2mg/L+IBA1.0mg/L+30g/L蔗糖。

关 键 词:何首乌  组织培养  正交设计
文章编号:1000-2642(2006)05-0756-03
收稿时间:06 30 2006 12:00AM
修稿时间:2006-06-30

OPTIMIZATION OF THE CULTURE CONDITIONS FOR THE ROOTING OF POLYGONUM MULTIFLORUM PLANTLETS
CHEN Ju, CHEN Guo - hui.OPTIMIZATION OF THE CULTURE CONDITIONS FOR THE ROOTING OF POLYGONUM MULTIFLORUM PLANTLETS[J].Journal of Southwest Agricultural University,2006,28(5):756-758.
Authors:CHEN Ju  CHEN Guo - hui
Institution:Agronomy and Biotechnology College, Southwest University, Chongqing 400716, China
Abstract:In an experiment with the orthogonal design,young shoots of Polygonum multiflorum Thunb were cultured on MS or 1/2 MS medium containing different concentrations of IBA,saccharose and NAA to induce rooting of the explants.Rooting induction was significantly affected by the concentrations of MS and IBA,followed by saccharose and NAA.The optimum combination of the culture condition was(1/2) MS + NAA 0.2 mg/L + IBA 1.0 mg/L + saccharose 30 g/L.
Keywords:Polygonum multiflorum Thunb  tissue culture  orthogonal design
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