Three-dimensional structure of recombinant human interferon-gamma |
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Authors: | S E Ealick W J Cook S Vijay-Kumar M Carson T L Nagabhushan P P Trotta C E Bugg |
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Institution: | Department of Pharmacology, University of Alabama, Birmingham 35294. |
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Abstract: | The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions. |
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