| 孝顺竹组培苗增殖培养影响因素探讨 |
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| 引用本文： | 李佳,马晓月,张学英,高志民.孝顺竹组培苗增殖培养影响因素探讨[J].世界竹藤通讯,2016,14(4):5. |
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| 作者姓名： | 李佳 马晓月 张学英 高志民 |
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| 作者单位： | 1 河北农业大学园艺学院 河北保定 071001;2 国际竹藤中心 国家林业局竹藤科学与技术重点开放实验室 北京 100102 |
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| 基金项目： | 林业公益性行业科研专项(编号:201504106)。 |
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| 摘 要： | 以孝顺竹组培苗为试材,研究了基本培养基、植物生长调节剂配比以及接种方式等对孝顺竹组培苗增殖培养的影响。结果表明,适宜孝顺竹增殖生长的培养基配方为MS+6-BA 2.0 mg/L+NAA 0.4 mg/L+活性炭1 g/L;对孝顺竹组培苗进行埋节处理可以提高增殖系数;同时孝顺竹组培苗去除顶梢后伤口下部竹节增大,有利于出现不定根,也可以提高增殖系数。
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| 关 键 词： | 孝顺竹 组培苗 植物生长调节剂 增殖 |
Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro |
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| Li Jia,Ma Xiaoyue,Zhang Xueying,Gao Zhimin.Influencing Factors to Proliferation Culture of Bambusa multiplex Plantlets in vitro[J].World Bamboo and Rattan,2016,14(4):5. |
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| Authors: | Li Jia Ma Xiaoyue Zhang Xueying Gao Zhimin |
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| Institution: | 1 College of Horticulture, Agricultural University of Hebei, Baoding 071001, Hebei, China;2 International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China |
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| Abstract: | The in vitro plantlets of Bambusa multiplex were selected as test materials to study the effects of the basic media, plant growth regulators and the inoculation methods on the plantlet proliferation culture. The results indicated that the suitable medium formula for the plantlet proliferation culture was MS + 6-BA 2. 0 mg/L + 0. 4 mg/L NAA + activated carbon 1 g/L. The treatment of burying internodes into medium could increase the proliferation coefficient of the bamboo plantlets. Meanwhile, the removal of plantlet top could contribute to the enlargement of the nodes at lower parts, and thus induce the formation of adventitious roots, which could also lead to the improvement of proliferation coefficient. |
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| Keywords: | Bambusa multiplex plantlets in vitro plant growth regulator proliferation |
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