| 产几丁质酶链霉菌SO1菌株的筛选及培养条件 |
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| 引用本文： | 杨文博,张历.产几丁质酶链霉菌SO1菌株的筛选及培养条件[J].天津农业科学,1997,3(3):1-4. |
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| 作者姓名： | 杨文博 张历 |
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| 作者单位： | [1]南开大学微生物学系 [2]天津市黄瓜研究所 |
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| 摘 要： | 从土样中分离获得的链霉菌ＳＯ１菌株在产酶培养基中，２８℃振荡培养７天，其胞外几丁质酶活力可达１８０９ｕ／ｍｌ。实验表明，不同碳、氮源及诱导物对ＳＯ１菌株产几丁质酶有不同的影响，以酵母粉对产酶的促进作用最为明显，比对照提高了７５．２％。部分脱乙酰几丁质、胶体几丁质及几丁质均可作为ＳＯ１菌株产几丁质酶的诱导物，其中以几丁质诱导产酶的效果最好。
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| 关 键 词： | 链霉菌 几丁质酶 筛选 培养基 |
STUDY ON THE SCREENING AND CULTURE CONDITION OF STRPTOMYCES SP. SO1 STRAIN |
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| Abstract: | The chitinase activity of Strptomyces sp.SO1 which was obtained from soil could reach 1809u/ml after SO1 was cultivated in the shaker for 7 days at the temperature of 28℃.It was showed that different inducers,carbon and nitrogen sources had different impacts on SO1 strain.Yeast powder was the most distinctive factor,which enhanced the enzyme activity by 75.2% compared with the comparison.Partial deacetyl chitin,collodial chitin could be used as the inducers of SO1 strain for the production of chitinase.Among them,chitin was the best inducer. |
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| Keywords: | Strptomyces sp SO1 strain chitinase culture condition |
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