| 高效聚磷菌株GM1的分离和聚磷特性研究 |
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| 引用本文： | 蔡天明,管莉菠,崔中利,李顺鹏.高效聚磷菌株GM1的分离和聚磷特性研究[J].土壤学报,2005,42(4):635-641. |
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| 作者姓名： | 蔡天明 管莉菠 崔中利 李顺鹏 |
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| 作者单位： | 南京农业大学生命科学学院微生物系,南京农业大学农业部环境微生物重点开放实验室,南京,210095 |
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| 基金项目： | 江苏省环境保护厅项目(2004007)、江苏省社会发展项目(BS2003028)资助 |
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| 摘 要： | 采用纯培养结合蓝白斑筛选法从土壤中筛选到一株高效聚磷菌,初步鉴定为费氏柠檬酸杆菌属(Citrobacterfreundii),命名为GM1。GM1在LB、YG和MOPS培养基上均可正常生长,其生长pH在5·5至8·5之间,最适生长pH为7·5。该菌在不同的培养基上最适生长温度不同,在LB培养基上为37℃,YG和MOPS-葡萄糖培养基上为30℃。在好氧条件下MOPS培养基培养24h后,GM1菌体含磷量为11·5%;上清液磷浓度由43·8mgL-1下降为14·7mgL-1,磷去除率达69%,poly-P染色显示菌体中有异染粒。GM1具有较强的聚磷能力。
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| 关 键 词： | 聚磷菌分离 筛选 菌体含磷量 聚磷特性 |
| 收稿时间： | 2004/11/22 0:00:00 |
| 修稿时间： | 2005/3/28 0:00:00 |
ISOLATION AND CHARACTERIZATION OF STRAIN GM1 WITH HIGH CAPABILITY OF ACCUMULATING POLY-P |
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| Cai Tianming,Guan Libo,Cui Zhongli and Li Shunpeng.ISOLATION AND CHARACTERIZATION OF STRAIN GM1 WITH HIGH CAPABILITY OF ACCUMULATING POLY-P[J].Acta Pedologica Sinica,2005,42(4):635-641. |
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| Authors: | Cai Tianming Guan Libo Cui Zhongli and Li Shunpeng |
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| Institution: | Department of Microbiology, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China;Department of Microbiology, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China;Department of Microbiology, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China;Department of Microbiology, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China |
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| Abstract: | |
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| Keywords: | PAO Isolation Screening Phosphorus concentration Accumulating phosphate characteristic |
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