| SSR分子标记方法鉴定杂交向日葵品种纯度研究 |
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| 引用本文： | 孟全业,赵建宗,李巧英,续建国,林展,王圆荣. SSR分子标记方法鉴定杂交向日葵品种纯度研究[J]. 山西农业科学, 2008, 36(5): 42-44 |
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| 作者姓名： | 孟全业 赵建宗 李巧英 续建国 林展 王圆荣 |
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| 作者单位： | 农业部农作物种子质量监督检验测试中心,山西,太原,030006;农业部全国农作物种子质量监督检验测试中心,北京,100026 |
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| 摘 要： | 通过对向日葵总DNA提取方法的比较、PCR扩增反应体系和程序的筛选、电泳检测方法的优化,以15个杂交组合为材料,从165对引物中筛选出扩增带型稳定、多态性丰富的8对核心引物。最终建立了一套适用于杂交向日葵品种纯度鉴定的技术体系。
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| 关 键 词： | 向日葵 SSR分子标记 品种纯度鉴定 |
| 文章编号： | 1002-2481(2008)05-0042-03 |
| 修稿时间： | 2008-02-26 |
Identification for Hybrid Sunflower Varietal Purity with SSR Molecular Marker |
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| MENG Quan-ye,ZHAO Jian-zong,LI Qiao-ying,XU Jian-guo,LIN Zhan,WANG Yuan-rong. Identification for Hybrid Sunflower Varietal Purity with SSR Molecular Marker[J]. Journal of Shanxi Agricultural Sciences, 2008, 36(5): 42-44 |
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| Authors: | MENG Quan-ye ZHAO Jian-zong LI Qiao-ying XU Jian-guo LIN Zhan WANG Yuan-rong |
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| Abstract: | Through comparision of sunflower DNA extraction method,screening for PCR amplification reaction system,program,and optimization for electrophoretic test methods,15 hybridized combinations were used as material,8 pairs core primers with stable amplifier band,rich polymorhism have been screened,and finally a set of purity identification technology system for sunflower has been set up. |
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| Keywords: | Sunflower SSR molecular marker Identification of varietal purity |
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