丝素膜表面接枝肝素分子的反应条件与体外抗凝血作用

利用等离子体处理技术使丝素膜表面被激活,以戊二醛作为交联剂,接枝肝素分子进行丝素膜表面抗凝血性修饰,从而为新型抗凝血生物医用材料的设计及临床应用提供试验依据。单因素优化实验结果显示:经等离子体和戊二醛处理后的丝素膜与肝素溶液的较好反应条件为pH 1.5、40℃、4 h,该条件下丝素膜表面肝素含量为37.62μg/g。经体外凝血试验检测,接枝肝素分子后的丝素膜具有较强的抗凝血活性;经稳定性试验分析,肝素与丝素膜结合稳定牢固不易被洗脱,提示其结合方式为共价键结合。

Reaction Condition of Fibroin Membrane Surface Grafting With Heparin and Anticoagulating Effect in vitro

Fibroin membrane surface was actived by the plasma processing,and then grafted with heparin via glutaraldehyde,from which the fibroin membrane has anticoagulating ability.The test provides the basis for the clinical practice and the design of new anticoagulating material for medical use.The result from single factor optimization showed that the better reaction condition for heparin and fibroin membrane,processed by the plasma and glutaraldehyde,was pH 1.5,temperature 40 ℃,time 4 hours.Under this condition the heparin content was 37.62 μg/g in fibroin membrane surface.Coagulant test in vitro showed that the fibroin membrane grafted with heparin has good anticoagulating;The compound was stable and not easily to be eluted in the stability test,suggesting that heparin was grafted with the fibroin membrane by covalent bond.