| 根癌农杆菌介导的转双价抗虫基因(CryIA+CpTI)苎麻 |
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| 引用本文： | 马雄风,喻春明,唐守伟,郭三堆,张锐,王延周,朱爱国,朱四元,熊和平. 根癌农杆菌介导的转双价抗虫基因(CryIA+CpTI)苎麻[J]. 作物学报, 2010, 36(5): 788-793. DOI: 10.3724/SP.J.1006.2010.00788 |
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| 作者姓名： | 马雄风 喻春明 唐守伟 郭三堆 张锐 王延周 朱爱国 朱四元 熊和平 |
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| 作者单位： | 1中国农业科学院麻类研究所/农业部麻类遗传改良与工程微生物重点开放实验室,湖南长沙410205;2中国农业科学院生物技术研究所,北京100081;3中国农业科学院棉花研究所,河南安阳455000 |
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| 基金项目： | 国家科技支撑计划，国家公益性行业(农业)科研专项 |
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| 摘 要： | 利用苎麻下胚轴高频再生体系，将携带人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因的植物表达载体pGBI4ABC转化到苎麻主栽品种中苎1号中。经根癌农杆菌侵染和共培养后，用50 mg L–1卡那霉素+ 300 mg L–1头孢霉素筛选共获得32株抗性植株; PCR检测显示26株为阳性, 占80%。Southern杂交结果证实, 外源基因已经整合到苎麻的基因组中。这项研究为最终创造兼抗鳞翅目及鞘翅目等害虫的苎麻种质材料奠定了基础。
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| 关 键 词： | 苎麻 抗虫基因 根癌农杆菌 转基因植株 |
| 收稿时间： | 2009-08-26 |
Transgenic of Ramie with Synthetic CryIA+CpTI Gene by Agrobacterium tume-faciens-Mediated |
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| MA Xiong-Feng,YU Chun-Ming,TANG Shou-Wei,GUO San-Dui,ZHANG Rui,WANG Yan-Zhou,ZHU Ai-Guo,ZHU Si-Yuan,XIONG He-Ping. Transgenic of Ramie with Synthetic CryIA+CpTI Gene by Agrobacterium tume-faciens-Mediated[J]. Acta Agronomica Sinica, 2010, 36(5): 788-793. DOI: 10.3724/SP.J.1006.2010.00788 |
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| Authors: | MA Xiong-Feng YU Chun-Ming TANG Shou-Wei GUO San-Dui ZHANG Rui WANG Yan-Zhou ZHU Ai-Guo ZHU Si-Yuan XIONG He-Ping |
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| Affiliation: | 1.Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences/Key Laboratory of Genetic Improvement & Engineering Microbiology for Bast Fiber Crops,Changsha 420105,China;2.Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081 China;3.Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,China |
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| Abstract: | Ramie [Boehmeria nivea L.(Guad.)] is a perennial herbaceous plant of the urticaceae family.Ramie can be affected by many insect pests above and below the ground during the growing season.The pests damage has become an important factor affecting ramie yield and quality in China.Application of chemical insecticides not only is less effective and costly,but also causes environmental pollution.Development of a genetic transformation system to introduce desired genes (e.g.toxin genes) into ramie could improve it... |
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| Keywords: | Ramie Insect resistant genes Agrobacterium tumefaciens Transegenetic plants |
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