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紫心甘薯二氢黄酮醇4-还原酶基因表达及酶活性与花色苷积累的相关性
引用本文:郭晋雅,李云萍,傅玉凡,黄成涛,周伟,高峰.紫心甘薯二氢黄酮醇4-还原酶基因表达及酶活性与花色苷积累的相关性[J].中国农业科学,2011,44(8):1736-1744.
作者姓名:郭晋雅  李云萍  傅玉凡  黄成涛  周伟  高峰
作者单位:1. 西南大学生命科学学院; 2. 华南师范大学;
基金项目:国家自然科学基金,广东省自然科学基金
摘    要: 【目的】以块根着色部位和程度不同的2个紫心甘薯品种(系)‘A5’和‘山川紫’,以及白心甘薯品种‘禺北白’为试材,研究了紫心甘薯二氢黄酮醇4-还原酶(DFR)基因表达及其酶活性与花色苷积累的相关性。【方法】对紫心甘薯在不同生长时期各器官(叶、茎、块根)的花色苷含量和DFR的活性进行了测定,对二者之间的变化趋势进行了相关性分析。此外,通过实时荧光定量PCR检测了‘A5’、‘山川紫’和‘禺北白’块根中IbDFR基因的表达量,以及不同发育时期的山川紫块根中IbDFR基因的表达量,并测定了相应的花色苷含量变化。【结果】(1)在同一生长时期的各品种(系)甘薯中,着色程度深的器官,其花色苷的含量较高,DFR活性也较高,不同器官的DFR活性与相应的花色苷含量呈显著线性正相关;(2)在不同的生长时期,3个品种(系)甘薯各器官的花色苷含量与DFR活性的变化趋势相一致,并且呈显著线性正相关;(3)3个品种(系)甘薯中,着色程度深的块根,其花色苷的含量较高,IbDFR基因的表达量也较高;(4)在山川紫块根的3个发育阶段,随着根的膨大,花色苷含量逐渐升高,IbDFR基因的表达量也逐渐增大。【结论】在不同生长时期的甘薯各器官中DFR活性与花色苷含量均呈线性正相关,且紫心甘薯块根中IbDFR基因表达量的变化与其花色苷含量的变化趋势相一致,说明:DFR是紫心甘薯花色苷合成代谢过程中的关键酶;紫心甘薯花色苷是原位合成的。

关 键 词:紫心甘薯  花色苷  二氢黄酮醇4-还原酶  相关性分析
收稿时间:2010-11-19;

Correlations Among the Enzyme Activity and Gene Expression of Dihydroflavonol 4-Reductase and Anthocyanin Accumulation in Purple-Fleshed Sweet Potato
GUO Jin-ya,LI Yun-ping,FU Yu-fan,HUANG Cheng-tao,ZHOU Wei,GAO Feng.Correlations Among the Enzyme Activity and Gene Expression of Dihydroflavonol 4-Reductase and Anthocyanin Accumulation in Purple-Fleshed Sweet Potato[J].Scientia Agricultura Sinica,2011,44(8):1736-1744.
Authors:GUO Jin-ya  LI Yun-ping  FU Yu-fan  HUANG Cheng-tao  ZHOU Wei  GAO Feng
Institution:GUO Jin-ya1,LI Yun-ping1,FU Yu-fan1,2,HUANG Cheng-tao3,ZHOU Wei3,GAO Feng3(1School of Life Science,Southwest University,Chongqing 400715,2Chongqing Sweet Potato Engneering and Technology Center,3School of Life Science,South China Normal University,Guangzhou 510631)
Abstract:【Objective】Two purple-fleshed sweet potato strains ‘A5’, ‘Yamakawamurasaki’ and one white-fleshed sweet potato strain ‘Yubeibai’ were used to study the role of Dihydroflavonol 4-reductase (DFR) in anthocyannin accumulation of purple- fleshed sweet potato. 【Method】Anthocyanin contents, DFR activities and expression of IbDFR gene were determined respectively, while the correlation between the contents of anthocyanin and DFR activities, and that between anthocyanin contents and IbDFR gene expression were analyzed. 【Result】The results show that the activities of DFR are dramatically and positively correlated with the content of anthocyanin no matter in different organs of the same strain or in same organ of the same strain in different growth stages. Moreover, the variation trends of the relative expression of IbDFR gene in storage roots of different strains and of different developing states of ‘Yamakawamurasaki’ also coincided with that of anthocyanin contents in corresponding storage roots. 【Conclusion】 These results indicate that DFR is a key enzyme in anthocyanin synthesis of purple- fleshed sweet potato, and the biosynthesis of anthocyanin is in situ synthesis in purple-fleshed sweet potato.
Keywords:purple-fleshed sweet potato  anthocyanin  dihydroflavonol 4-reductase  correlation analysis  
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