三角紫叶酢浆草叶色变异株系的RAPD和ISSR标记初步鉴定

 以三角紫叶酢浆草叶色变异株系和其野生型植株为材料，对其遗传物质进行分子水平上的初步检测。采用DNA混合样本池法，构建变异型样本池和野生型样本池，并以这两个DNA池做模板分别进行RAPD-PCR和ISSR-PCR 扩增。从70个RAPD随机引物和100个ISSR随机引物中分别筛选出2个RAPD特异引物（S76，S118）和2个ISSR特异引物（UBC818，UBC868），均能在上述两个DNA池之间扩增出稳定的差异性条带共6条。其中，变异株系的差异性缺失带S118 700-800测序成功，其序列包含编码叶绿体光系统Ⅰ第Ⅶ亚基的基因。研究结果表明，与其野生型相比，该叶色变异株系的确发生了遗传物质的变化，且在一定继代范围内能通过无性繁殖方式稳定存在。

Preliminary Identification of Leaf Color Mutational Strain from Oxalis triangularis Using RAPD and ISSR Markers

A leaf color mutational strain and the wild type of Oxalis triangularis were identified preliminarily at the molecular level. DNA-pooling method was used in this study to establish the DNA-pools of the wild type and the mutational type,respectively. These DNA-pools were used as templates for RAPD-PCR and ISSR-PCR,respectively. Two specific RAPD primers,i.e.,S76 and S118,and two specific ISSR primers,i.e.,UBC818 and UBC868,were selected from 70 RAPD primers and 100 ISSR primers,respectively,producing 6 speci...