Plasmid profile analysis of salmonellae in a large-animal hospital |
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| Authors: | H S Rumschlag J R Boyce |
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| Affiliation: | 1. Governmental Institute of Public Health of Lower Saxony (Niedersächsisches Landesgesundheitsamt, NLGA), Hanover, Germany;2. Postgraduate Training for Applied Epidemiology (PAE), Robert Koch Institute, Berlin, Germany; affiliated with the European Programme for Intervention Epidemiology Training, ECDC, Sweden;3. District Stade, Local Health Authority, Stade, Germany;4. Austrian Agency for Health and Food Safety (AGES), Graz, Austria;5. National Reference Centre for Salmonella and other Bacterial Enteritic Pathogens, Division of Enteropathogenic Bacteria and Legionella (FG11), Robert Koch Institute, Wernigerode, Germany;6. NRW Centre for Health, Bochum, Germany;1. Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain;2. Department of Food Engineering, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203, Cartagena, Murcia, Spain;1. Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, 261 Chemdan-gwagiro, Gwangju 500-712, Republic of Korea;2. Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Chemdan-gwagiro, Gwangju 500-712, Republic of Korea;3. Department of Agricultural Chemistry and Food Science and Technology, Gyeongsang National University, 900 Gajwa-dong, Jinju Gyeongnam 660-701, Republic of Korea |
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| Abstract: | The plasmid profiles of salmonellae isolated from the patients and environment of the Purdue Large Animal Hospital were used as markers to identify strains and to assess the impact of improvements in hospital operation on nosocomial salmonellosis. Plasmid profile analysis proved to be more sensitive than either serotyping or antimicrobial susceptibility testing in identifying Salmonella isolates. During June and July 1983, 29 of 34 salmonellae isolated were one of three strains with distinct plasmid profiles: one S. typhimurium var. Copenhagen, and two S. muenchen. Each of these strains was isolated from at least one patient and two environmental sites, suggesting the possibility that infections were hospital-acquired. Patient and environmental sampling was repeated in June and July 1984, after improvements had been made in hospital traffic flow and sanitation. In contrast to 1983, only seven isolates, representing six strains not seen previously, were obtained in 1984. None of these strains was isolated from both patient and environmental sources. The results indicate that the high incidence of clinical salmonellosis in 1983 was largely due to nosocomial infections. The decrease in the incidence of salmonellosis and the absence of the 1983 strains from samples taken in 1984 were presumed to be due to improvements made in hospital operation. This study demonstrates the value of plasmid analysis in monitoring nosocomial salmonellosis in a veterinary hospital. |
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