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| 鸡MHC基因B-G区域的生物信息学分析 | |
| 引用本文： | 余春林,徐亚欧,杜华锐,李小成,辅宏璞,蒋小松.鸡MHC基因B-G区域的生物信息学分析[J].四川畜牧兽医,2010,37(5):26-28. |
| 作者姓名： | 余春林 徐亚欧 杜华锐 李小成 辅宏璞 蒋小松 |
| 作者单位： | 1. 西南民族大学生命科学与技术学院,四川,成都,610041;四川省畜牧科学研究院,四川,成都,610066 2. 西南民族大学生命科学与技术学院,四川,成都,610041 3. 四川省畜牧科学研究院,四川,成都,610066 |
| 基金项目： | 西南民族大学研究生创新型科研项目，国家科技部863计划重点项目 |
| 摘 要： | 根据鸡主要组织相容性复合体（MHC）基因B-G区域外显子2的序列设计引物,采用直接测序的方法对该区域（207bp）进行序列测定,并对所获得核苷酸序列进行生物信息学分析。结果显示：该片段中碱基T、C、A、G的平均含量分别为22.0%、19.8%、24.8%、33.4%,C＋G含量为53.2%,T＋A含量为46.8%,C＋G含量高于T＋A含量;同时检测到27个核苷酸变异位点,导致14个氨基酸位点的变异;16条序列进行单倍型构建获得16种单倍型,单倍型多样度达到了高的多样水平。试验结果表明,鸡MHC-B-F基因外显子2的序列表现出了高度的多态性,而且其序列的多态性更多地表现在氨基酸水平上。 |
| 关 键 词： | MHC B—G基因 序列分析 鸡 |
Bioinformatics analysis of chicken major hiscompatibility complex B-G gene |
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| YU Chun-lin,XU Ya-ou,DU Hua-rui,et al..Bioinformatics analysis of chicken major hiscompatibility complex B-G gene[J].Sichuan Animal & Veterinary Sciences,2010,37(5):26-28. | |
| Authors: | YU Chun-lin XU Ya-ou DU Hua-rui |
| Institution: | YU Chun-lin1,2,XU Ya-ou1,DU Hua-rui2,et al. (1.College of Life Science , Technology,Southwest University for Nationalities,Sichuan Chengdu 610041,China,2.Sichuan Animal Science Academy,Sichuan Chengdu 610066,China) |
| Abstract: | The chicken major hiscompatibility complex(MHC) B-G gene exon 2 was amplified and sequenced. The sequenced data were analyzed by using bioinformatics software. The result showed that the average content of T,C,A and G was 22.0% ,19.8% ,24.8% and 33.4% respectively. The content of T+A was 46.8%,which was lower than that of C+G(53.2%). 27 nucleotide mutational loci were detected,which caused 14 amino acid mutational sites. 16 haplotypes were found based on sequences of 16 individuals,and the diversity of hapl... |
| Keywords: | MHC |
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