| 碳源和氮源对黑曲霉产木聚糖酶的影响 |
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| 引用本文： | 白爱枝,鲍秀珍,梁运章,潘仁瑞.碳源和氮源对黑曲霉产木聚糖酶的影响[J].饲料工业,2006,27(2):17-20. |
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| 作者姓名： | 白爱枝 鲍秀珍 梁运章 潘仁瑞 |
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| 作者单位： | 1. 内蒙古自治区离子束生物工程重点实验室,010021,呼和浩特市大学西路235号,内蒙古大学理工学院
2. 合肥学院生物科学与技术系 |
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| 基金项目： | 内蒙古专项基金;中国科学院资助项目 |
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| 摘 要： | 研究了碳源、氮源以及其它因子对黑曲霉(Aspergillusniger)A3菌株产木聚糖酶的影响。结果表明,当迟效碳源为玉米芯70g/l、速效碳源为葡萄糖10g/l,氮源为NaNO33g/l和(NH4)2SO43g/l,麸皮5g/l,用无氮的Mandels无机盐溶液配制,起始pH值5.5～6.0,并添加中和剂CaCO3调控发酵pH值,250ml三角瓶装液量为30ml时,黑曲霉A3液体发酵酶活力达405.6IU/ml。采用玉米芯代替半纤维素作为主要碳源,可以大大降低生产成本,减少环境污染,有利于工业化生产。
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| 关 键 词： | 碳源 氮源 木聚糖酶 液体发酵 |
| 收稿时间： | 2005-10-22 |
| 修稿时间： | 2005-10-22 |
Effects of carbon source and nitrogen source on xylanase production by Aspergillus niger A3 in submerged fermentation |
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| Bai Aizhi,Bao Xiuzhen,Liang Yunzhang,Pan Renrui.Effects of carbon source and nitrogen source on xylanase production by Aspergillus niger A3 in submerged fermentation[J].Feed Industry,2006,27(2):17-20. |
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| Authors: | Bai Aizhi Bao Xiuzhen Liang Yunzhang Pan Renrui |
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| Abstract: | Effects of carbon source,nitrogen source and other factors on xylanase production of Aspergillus niger A3 were studied. The appropriate medium composition was as follows:corn-core 70g/l,NaNO3 3g/l and (NH4)2SO4 3g/l,wheat bran 5g/l with nitrogen-free Mandels mineral salt solution,initial pH5.4~6.0,Adjusting pH by neutralization agent H . When 3ml(~107/ml) spores were inoculated into 30ml medium in 250ml Erlenmeyer flask ,and it cultivated in shaker at 28℃ for 72h,the xylanase activity of culture filtrate reached 405. 6 IU/ml. Using corn-core could reduse the cost,decrease the environment pollution and might be applied to industrial production. |
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| Keywords: | carbon source nitrogen souree xylanase submerged of xylanase fermentation |
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